USMG5 Polyclonal antibody

USMG5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17716-1-AP

Synonyms

HCV F transactivated protein 2, HCVFTP2, USMG5

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Validation Data Gallery

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  WB analysis using 17716-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 17716-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17716-1-AP (USMG5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17716-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17716-1-AP (USMG5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human liver tissue, human brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

17716-1-AP targets USMG5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USMG5 fusion protein Ag12086
• Full Name: up-regulated during skeletal muscle growth 5 homolog (mouse)
• Calculated Molecular Weight: 58 aa, 7 kDa
• Observed Molecular Weight: 6 kDa
• GenBank Accession Number: BC072683
• Gene Symbol: USMG5
• Gene ID (NCBI): 84833
• RRID: AB_2214288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	J Clin Invest; Deficiency in Kelch protein Klhl31 causes congenital myopathy in mice.; Authors - James B Papizan
mouse	IF	Sci Rep; miR-379 links glucocorticoid treatment with mitochondrial response in Duchenne muscular dystrophy.; Authors - M Sanson
rat	WB	Mol Psychiatry; Proteasome-independent K63 polyubiquitination selectively regulates ATP levels and proteasome activity during fear memory formation in the female amygdala; Authors - Kayla Farrell