USP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

33

Cat no : 14346-1-AP

Synonyms

Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, N-terminal fragment, Ubiquitin carboxyl-terminal hydrolase 1, hUBP, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis of HEK-293 using 14346-1-AP

  WB result of USP1 antibody (14346-1-AP, 1:1000) with si-Control and si-USP1 transfected HEK293 cells.

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  WB analysis using 14346-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14346-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14346-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14346-1-AP (USP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14346-1-AP

  IP result of anti-USP1 (IP:14346-1-AP, 4ug; Detection:14346-1-AP 1:4000) with HEK-293 cells lysate 1800 ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 10 publications below
• WB: See 31 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• IP: See 8 publications below
• CoIP: See 2 publications below

Product Information

14346-1-AP targets USP1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP1 fusion protein Ag5686
• Full Name: ubiquitin specific peptidase 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110 kDa, 100 kDa
• GenBank Accession Number: BC050525
• Gene Symbol: USP1
• Gene ID (NCBI): 7398
• RRID: AB_2214314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. It is also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases (PMID: 9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment (PMID: 18082604).

Publications

Species	Application	Title
human	WB	Nat Chem Biol; A selective USP1-UAF1 inhibitor links deubiquitination to DNA damage responses.; Authors - Qin Liang
human	WB	Mol Cell; Polyubiquitinated PCNA recruits the ZRANB3 translocase to maintain genomic integrity after replication stress.; Authors - Ciccia Alberto A
human	WB,IF	Nat Commun; The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites.; Authors - Kyungsoo Ha
human	WB	Nat Commun; hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma.; Authors - Pauline Herviou
human	WB	Cancer Immunol Res; USP22 deubiquitinates CD274 to suppress anti-cancer immunity.; Authors - Xing Huang
human	WB,IHC,IF,IP	Theranostics; CRISPR/Cas9-based genome-wide screening for deubiquitinase subfamily identifies USP1 regulating MAST1-driven cisplatin-resistance in cancer cells; Authors - Apoorvi Tyagi