USP1 Monoclonal antibody, PBS Only

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  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  WB result of USP1 antibody (66069-1-Ig, 1:2000) with si-Control and si-USP1 transfected HepG2 & HEK293 cells. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66069-1-Ig (same clone as 66069-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66069-1-Ig (same clone as 66069-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66069-1-Ig (same clone as 66069-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66069-1-Ig (same clone as 66069-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66069-1-Ig (USP1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66069-1-Ig (USP1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66069-1-Ig (same clone as 66069-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 66069-1-Ig(USP1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66069-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66069-1-PBS

Clone No.1C10H6

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1C10H6, Deubiquitinating enzyme 1, EC:3.4.19.12, hUBP, Ubiquitin carboxyl-terminal hydrolase 1

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Product Information

66069-1-PBS targets USP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5904

  Product name: Recombinant human USP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 437-785 aa of BC050525

  Sequence: RTRCLECESLTERREDFQDISVPVQEDELSKVEESSEISPEPKTEMKTLRWAISQFASVERIVGEDKYFCENCHHYTEAERSLLFDKMPEVITIHLKCFAASGLEFDCYGGGLSKINTPLLTPLKLSLEEWSTKPTNDSYGLFAVVMHSGITISSGHYTASVKVTDLNSLELDKGNFVVDQMCEIGKPEPLNEEEARGVVENYNDEEVSIRVGGNTQPSKVLNKKNVEAIGLLGGQKSKADYELYNKASNPDKVASTAFAENRNSETSDTTGTHESDRNKESSDQTGINISGFENKISYVVQSLKEYEGKWLLFDDSEVKVTEEKDFLNSLSPSTSPTSTPYLLFYKKL

• Full Name: ubiquitin specific peptidase 1
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC050525
• Gene Symbol: USP1
• Gene ID (NCBI): 7398
• RRID: AB_11043340
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94782
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP1(Ubiquitin carboxyl-terminal hydrolase 1) is a negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2.It is Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA.This protein, which consists of 785 amino acids with a deduced molecular mass of 88.2 kDa, possesses His and Cys domains that are highly conserved in all members of the ubiquitin-specific processing (UBP) family of proteases(PMID:9806842). The USP1 enzyme can undergo autocleavage, resulting in a 100 kDa N-terminal fragment and a 14 kDa C-terminal fragment(PMID:18082604).