USP10 Polyclonal antibody

USP10 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 19374-1-AP

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10



Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19374-1-AP is USP10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP10 fusion protein Ag7233
Full Name ubiquitin specific peptidase 10
Calculated molecular weight 87 kDa, 92 kDa
Observed molecular weight 100-130 kDa
GenBank accession numberBC000263
Gene symbol USP10
Gene ID (NCBI) 9100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

Protocols

Product Specific Protocols
WB protocol for USP10 antibody 19374-1-APDownload protocol
IP protocol for USP10 antibody 19374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF, WB

Int J Mol Med

Isoquercitrin protects HUVECs against high glucose‑induced apoptosis through regulating p53 proteasomal degradation.

Authors - Libo Liu
humanIF

J Cell Biol

High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate.

Authors - Brian D Freibaum
humanWB

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
  • KD Validated
WB

Mol Cell

Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance.

Authors - Anna K Lee
human

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gonzalo (Verified Customer) (03-21-2018)

Great antibody for WB and IP

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
USP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HEK293 (Cat no:19374-1-AP)