Tested Applications
| Positive WB detected in | MCF-7 cells, HCT 116 cells, HEK-293 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19374-1-AP targets USP10 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7233 Product name: Recombinant human USP10 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 444-798 aa of BC000263 Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL Predict reactive species |
| Full Name | ubiquitin specific peptidase 10 |
| Calculated Molecular Weight | 87 kDa, 92 kDa |
| Observed Molecular Weight | 100-130 kDa |
| GenBank Accession Number | BC000263 |
| Gene Symbol | USP10 |
| Gene ID (NCBI) | 9100 |
| RRID | AB_10858617 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14694 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP10 antibody 19374-1-AP | Download protocol |
| IHC protocol for USP10 antibody 19374-1-AP | Download protocol |
| IP protocol for USP10 antibody 19374-1-AP | Download protocol |
| WB protocol for USP10 antibody 19374-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-USP10 antibody (Cat# 19374-1-AP) has 47 total citations published across 40 journals, including Cell, Nature Communications, Molecular Cancer, Molecular Cell, Advanced Science, Oncogene, and EMBO Reports. Associated research fields encompass oncology (pancreatic, breast, liver, bladder, and other cancers), ferroptosis, stress granule biology, cardiac disease, fibrosis, neurodegeneration, viral infection, drug resistance, and stem cell differentiation. Primary applications include WB, IP, IHC, IF, CoIP, and RIP.
| Species | Application | Title |
|---|---|---|
Cell G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules. | ||
Mol Cancer DCLK1 drives malignant progression and chemoresistance of bladder cancer by deubiquitinating HDAC6. | ||
Nat Commun Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.
| ||
Nat Commun FAD synthase confers ferroptosis resistance and restrains CD8+ T cell recruitment in hepatocellular carcinoma | ||
Redox Biol USP10 protects against pressure overload-induced mitochondrial morphofunctional defects and pathological cardiac hypertrophy through stabilizing cytoplasmic Mfn2
| ||
Mol Cell Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance. |
Reviews
What customers say
One reviewer rated this antibody 5 out of 5 stars. They used it for Western Blot and Immunoprecipitation on HEK293 cells at a 1:1000 dilution and described it as a "great antibody for WB and IP." Overall, the feedback is positive, highlighting its strong performance in both applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gonzalo (Verified Customer) (03-21-2018) | Great antibody for WB and IP
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