USP10 Polyclonal antibody

USP10 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 19374-1-AP

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, mouse tongue tissue
Positive IP detected inHeLa cells
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19374-1-AP is USP10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP10 fusion protein Ag7233
Full Name ubiquitin specific peptidase 10
Calculated molecular weight 87 kDa, 92 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC000263
Gene symbol USP10
Gene ID (NCBI) 9100
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.

Protocols

Product Specific Protocols
WB protocol for USP10 antibody 19374-1-APDownload protocol
IF protocol for USP10 antibody 19374-1-APDownload protocol
IP protocol for USP10 antibody 19374-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.

Authors - Hu Lei
  • KD Validated
WB

Mol Cell

Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance.

Authors - Anna K Lee
human

Cell

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors - Peiguo Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gonzalo (Verified Customer) (03-21-2018)

Great antibody for WB and IP

  • Applications: Western Blot,Immunoprecipitation,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293
USP10 Antibody Western Blot,Immunoprecipitation, validation (1:1000 dilution) in HEK293 (Cat no:19374-1-AP)