|Positive WB detected in||MCF-7 cells, HEK-293 cells|
|Positive IP detected in||HeLa cells|
|Positive IF detected in||HEK-293 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 19374-1-AP is USP10 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||USP10 fusion protein Ag7233|
|Full Name||ubiquitin specific peptidase 10|
|Calculated molecular weight||87 kDa, 92 kDa|
|Observed molecular weight||100-130 kDa|
|GenBank accession number||BC000263|
|Gene ID (NCBI)||9100|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.
G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.
Translational Repression of G3BP in Cancer and Germ Cells Suppresses Stress Granules and Enhances Stress Tolerance.
Cell Death Differ
Targeting USP10 induces degradation of oncogenic ANLN in esophageal squamous cell carcinoma
Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia.
J Cell Biol
High-fidelity reconstitution of stress granules and nucleoli in mammalian cellular lysate.
Br J Pharmacol
Limonin stabilises SIRT6 by activating USP10 in cardiac hypertrophy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gonzalo (Verified Customer) (03-21-2018)
Great antibody for WB and IP