USP10 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81435-2-RR (same clone as 81435-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 81435-2-RR (same clone as 81435-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81435-2-RR (USP10 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 81435-2-RR (same clone as 81435-2-PBS)

  WB result of USP10 antibody (81435-2-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP10 transfected MCF-7 cells. This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 81435-2-RR (same clone as 81435-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP10 antibody (81435-2-RR, Clone: 240583G6 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 81435-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP10 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 81435-2-PBS

Clone No.240583G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

240583G6, Deubiquitinating enzyme 10, EC:3.4.19.12, KIAA0190, Ubiquitin carboxyl-terminal hydrolase 10

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Product Information

81435-2-PBS targets USP10 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7233

  Product name: Recombinant human USP10 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 444-798 aa of BC000263

  Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL

• Full Name: ubiquitin specific peptidase 10
• Calculated Molecular Weight: 87 kDa, 92 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC000263
• Gene Symbol: USP10
• Gene ID (NCBI): 9100
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q14694
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP10, also named as USP32 and NY-REN-60, belongs to the peptidase C19 family.The deubiquitinating protease USP10 has been identified as a new regulator of p53 in the DNA damage response and tumor development.