USP11 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP11 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10244-1-AP

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11

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Validation Data Gallery

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  WB analysis of Jurkat using 10244-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 10244-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10244-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10244-1-AP (USP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10244-1-AP

  IP result of anti-USP11 (IP:10244-1-AP, 4ug; Detection:10244-1-AP 1:300) with HEK-293 cells lysate 1480ug.

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  IHC staining of human colon cancer using 10244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10244-1-AP (USP11 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, HEK-293 cells, human testis tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Product Information

10244-1-AP targets USP11 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP11 fusion protein Ag0296
• Full Name: ubiquitin specific peptidase 11
• Calculated Molecular Weight: 963 aa, 110 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC000350
• Gene Symbol: USP11
• Gene ID (NCBI): 8237
• RRID: AB_2288400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

Publications

Species	Application	Title
human	WB	Nat Cell Biol; XRCC1 protects transcription from toxic PARP1 activity during DNA base excision repair.; Authors - Marek Adamowicz
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB,IHC	Clin Transl Med; EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.; Authors - Hongyu Zhao
human	IHC	Oncogene; The deubiquitinase USP11 regulates cell proliferation and ferroptotic cell death via stabilization of NRF2 USP11 deubiquitinates and stabilizes NRF2.; Authors - Chunjie Meng
human	WB,IHC	Cancer Lett; The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.; Authors - Lijun Qiao ; KD Validated
human	WB	Oncotarget; Ubiquitin-specific protease 11 functions as a tumor suppressor by modulating Mgl-1 protein to regulate cancer cell growth.; Authors - Key-Hwan Lim ; KD Validated