• Featured Product
  • KD/KO Validated

USP11 Polyclonal antibody

USP11 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10244-1-AP

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11



Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, human testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10244-1-AP targets USP11 in WB, IP, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP11 fusion protein Ag0296
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC000350
Gene symbol USP11
Gene ID (NCBI) 8237
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

Protocols

Product Specific Protocols
WB protocol for USP11 antibody 10244-1-APDownload protocol
IHC protocol for USP11 antibody 10244-1-APDownload protocol
IP protocol for USP11 antibody 10244-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Clin Transl Med

EGFR-vIII downregulated H2AZK4/7AC though the PI3K/AKT-HDAC2 axis to regulate cell cycle progression.

Authors - Hongyu Zhao
humanWB

Biochem Biophys Res Commun

CRL4DCAF8 and USP11 oppositely regulate the stability of myeloid leukemia factors (MLFs).

Authors - Daoyuan Huang
  • KD Validated
humanWB

Am J Cancer Res

Ubiquitin-specific protease 11 (USP11) functions as a tumor suppressor through deubiquitinating and stabilizing VGLL4 protein.

Authors - Encheng Zhang
  • KD Validated
humanWB,IHC

Am J Cancer Res

Aberrant USP11 expression regulates NF90 to promote proliferation and metastasis in hepatocellular carcinoma.

Authors - Changmao Zhang
  • KD Validated
humanWB

Oncotarget

Ubiquitin-specific protease 11 functions as a tumor suppressor by modulating Mgl-1 protein to regulate cancer cell growth.

Authors - Key-Hwan Lim
  • KD Validated
humanWB,IHC

Cancer Lett

The E2F1/USP11 positive feedback loop promotes hepatocellular carcinoma metastasis and inhibits autophagy by activating ERK/mTOR pathway.

Authors - Lijun Qiao
  • KD Validated