USP11 Monoclonal antibody

USP11 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2G6E7

Cat no : 67805-1-Ig

Synonyms

Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11



Tested Applications

Positive WB detected inHCT 116 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67805-1-Ig targets USP11 in WB, IF,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP11 fusion protein Ag19058
Full Name ubiquitin specific peptidase 11
Calculated molecular weight 963 aa, 110 kDa
Observed molecular weight110 kDa
GenBank accession numberBC000350
Gene symbol USP11
Gene ID (NCBI) 8237
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.

Protocols

Product Specific Protocols
WB protocol for USP11 antibody 67805-1-IgDownload protocol
IF protocol for USP11 antibody 67805-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols