USP12 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12608-1-AP

Synonyms

Deubiquitinating enzyme 12, UBH1, Ubiquitin hydrolyzing enzyme 1, Ubiquitin thiolesterase 12, USP12, USP12L1

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Validation Data Gallery

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  WB analysis using 12608-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12608-1-AP (USP12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

12608-1-AP targets USP12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP12 fusion protein Ag3309
• Full Name: ubiquitin specific peptidase 12
• Calculated Molecular Weight: 370 aa, 43 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC026072
• Gene Symbol: USP12
• Gene ID (NCBI): 219333
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-specific peptidase 12 (USP12) belongs to the ubiquitin-specific protease (USP) family of deubiquitinases that are critical for ubiquitin signaling and protein quality control. USPs function as cysteine proteases to trim or remove ubiquitin conjugates from selected substrates. Deubiquitination is important for ubiquitin signaling-mediated processes, such as regulation of protein levels or activity. Depletion of USP12 suppressed cell growth and clonogenicity and inhibited autophagy(PMID: 30266909 34997217 35898171).

Publications

Species	Application	Title
mouse	WB	J Exp Med; USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.; Authors - Zhongxia Yu ; KD Validated