Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, A375 cells, HEK-293 cells, rat testis tissue, PC-3 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16840-1-AP targets USP13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10527 Product name: Recombinant human USP13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 515-863 aa of BC016146 Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS Predict reactive species |
| Full Name | ubiquitin specific peptidase 13 (isopeptidase T-3) |
| Calculated Molecular Weight | 863 aa, 97 kDa |
| Observed Molecular Weight | 97-100 kDa |
| GenBank Accession Number | BC016146 |
| Gene Symbol | USP13 |
| Gene ID (NCBI) | 8975 |
| RRID | AB_2214569 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92995 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP13 antibody 16840-1-AP | Download protocol |
| IHC protocol for USP13 antibody 16840-1-AP | Download protocol |
| IP protocol for USP13 antibody 16840-1-AP | Download protocol |
| WB protocol for USP13 antibody 16840-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USP13 Polyclonal antibody (Cat# 16840-1-AP) has accumulated 45 citations published across 36 journals, including Nature Communications, Oncogene, Cell Death & Differentiation, Advanced Science, and Redox Biology. Associated research fields encompass oncology (colorectal, gastric, lung, bladder, breast, and head-and-neck cancers), cardiology (hypertrophy, cardiomyopathy, cardiotoxicity), inflammation, fibrosis, ferroptosis, autophagy, and neurology. Tested applications include WB, IHC, IF, IP, and CoIP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Nat Commun Cardiomyocyte-derived USP13 protects hearts from hypertrophy via deubiquitinating and stabilizing STAT1 in male mice.
| ||
Redox Biol USP13 ameliorates myocardial infarction injury by inhibiting ferroptosis via stabilizing ALDOA.
| ||
Acta Pharm Sin B Deubiquitinase USP13 alleviates doxorubicin-induced cardiotoxicity through promoting the autophagy-mediated degradation of STING | ||
Adv Sci (Weinh) Intestinal Epithelial-Derived USP13 Alleviates Colonic Inflammation by Suppressing GRP78-mediated Endoplasmic Reticulum Stress.
| ||
Adv Sci (Weinh) PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer. | ||
Cell Death Differ USP13 depletion sensitizes colorectal cancer cells to necroptosis by destabilizing cIAP2 proteins.
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Reviews
What customers say
The single review for this antibody is a 5-star rating. The reviewer used it for Western Blot on MeT-5A cells at a 1:2000 dilution in 5% milk and reported it as a very good antibody, noting that its specificity was validated through USP13 knock-down. Overall, the feedback is positive, highlighting reliable performance and confirmed target specificity in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Francesca (Verified Customer) (05-22-2019) | Very good antibody for western blot. Antibody specificity validated by USP13 knock-down.
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