USP18 Polyclonal antibody, PBS Only

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• USP18 Polyclonal antibody, PBS Only (12153-1-PBS)

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  WB analysis of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

  WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 12153-1-AP (same clone as 12153-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12153-1-AP (USP18 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 12153-1-AP (same clone as 12153-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP18 antibody (12153-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12153-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12153-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

43 kDa ISG15-specific protease, EC:3.4.19.12, hUBP43, ISG15-specific-processing protease, ISG43

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Product Information

12153-1-PBS targets USP18 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2785

  Product name: Recombinant human USP18 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-372 aa of BC014896

  Sequence: MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDYPHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVPFQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNLIKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFDVDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQTLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGGQYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCTYGNPNYHWQETAYLLVYMKMEC

• Full Name: ubiquitin specific peptidase 18
• Calculated Molecular Weight: 372 aa, 43 kDa
• Observed Molecular Weight: 43 kDa, 70 kDa
• GenBank Accession Number: BC014896
• Gene Symbol: USP18
• Gene ID (NCBI): 11274
• RRID: AB_2877830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UMW8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).