USP19 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP19 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 25768-1-AP

Synonyms

Deubiquitinating enzyme 19, KIAA0891, Ubiquitin thiolesterase 19, USP19, ZMYND9

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Validation Data Gallery

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  WB analysis of Jurkat using 25768-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25768-1-AP

  WB result of USP19 antibody (25768-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP19 transfected HeLa cells.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25768-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25768-1-AP (USP19 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 25768-1-AP

  IP result of anti-USP19 (IP:25768-1-AP, 4ug; Detection:25768-1-AP 1:500) with Jurkat cells lysate 2400ug.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, Jurkat cells, MDA-MB-231 cells
• Positive IP detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below

Product Information

25768-1-AP targets USP19 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP19 fusion protein Ag22646
• Full Name: ubiquitin specific peptidase 19
• Calculated Molecular Weight: 1318 aa, 146 kDa
• Observed Molecular Weight: 70 kDa, 120 kDa, 150-180 kDa
• GenBank Accession Number: BC146752
• Gene Symbol: USP19
• Gene ID (NCBI): 10869
• RRID: AB_2713918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-specific protease (USP)19 is a recently identified deubiquitinating enzyme (DUB) having multiple splice variants and cellular functions. Since the sum of molecular weights of the two small bands (65 kDa+120 kDa) approximates the size of the full-length protein (~185 kDa), USP19 is likely to be endoproteolytically cleaved. Interestingly, the small bands were not observed when the DUB activity was inactivated, suggesting that the cleavage of USP19 depends on its DUB activity (PMID: 25088257).

Publications

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
	IHC	Clin Transl Med; Deubiquitinase USP19 modulates apoptotic calcium release and endoplasmic reticulum stress by deubiquitinating BAG6 in triple negative breast cancer; Authors - Xiaoqiang Zhang
human	WB,IHC,IP	Mol Ther; Dual role of deubiquitinating enzyme USP19 regulates mitotic progression and tumorigenesis by stabilizing survivin; Authors - Apoorvi Tyagi ; KD Validated
human	WB	J Cell Biol; USP19 promotes hypoxia-induced mitochondrial division via FUNDC1 at ER-mitochondria contact sites.; Authors - Peiyuan Chai
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran ; KD Validated
human	WB,IF,CoIP	Cell Biol Toxicol; Deubiquitinase USP19 enhances phenylalanine hydroxylase protein stability and its enzymatic activity.; Authors - Neha Sarodaya ; KO Validated