USP2 Polyclonal antibody

USP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15404-1-AP

Synonyms

Deubiquitinating enzyme 2, ubiquitin specific peptidase 2, Ubiquitin thiolesterase 2, UBP41, USP2, USP9

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Validation Data Gallery

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  WB analysis of mouse testis using 15404-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15404-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15404-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15404-1-AP (USP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 15404-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15404-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15404-1-AP (USP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 15404-1-AP

  Immunofluorescent analysis of HepG2 cells, using USP2 antibody 15404-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse testis tissue, MCF-7 cells, mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• CoIP: See 1 publications below

Product Information

15404-1-AP targets USP2 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP2 fusion protein Ag7658
• Full Name: ubiquitin specific peptidase 2
• Calculated Molecular Weight: 68 kDa
• Observed Molecular Weight: 41-50 kDa, 68 kDa
• GenBank Accession Number: BC002854
• Gene Symbol: USP2
• Gene ID (NCBI): 9099
• RRID: AB_10596634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin, a highly conserved protein involved in the regulation of intracellular protein breakdown, cell cycle regulation, and stress response, is released from degraded proteins by disassembly of the polyubiquitin chains. The disassembly process is mediated by ubiquitin-specific proteases (USPs). USP2 is also named as UBP41. USP2 can interact indirectly with multiple clock proteins, so it remains possible that USP2 may modulate the ubiquitination and function of these other proteins. It has 4 isoforms produced by alternative splicing with the MW of 68, 41, 40 and 45 kDa. USP2 can exsit as a homooligomer.

Publications

Species	Application	Title
human	WB,CoIP	Transl Oncol; The role of deubiquitinase USP2 in driving bladder cancer progression by stabilizing EZH2 to epigenetically silence SOX1 expression; Authors - Fanghua Xu ; KD Validated