USP21 Recombinant monoclonal antibody

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• USP21 Recombinant monoclonal antibody (87962-1-RR)

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  WB analysis using 87962-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87962-1-RR (USP21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

USP21 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87962-1-RR

Clone No.260026G9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

EC:3.4.19.12, PP1490, Ubiquitin carboxyl-terminal hydrolase 21, Ubiquitin thioesterase 21, Ubiquitin-specific-processing protease 21

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Tested Applications

• Positive WB detected in: HeLa cells, DU 145 cells, PC-3 cells, A549 cells, EC109 cells, RAW264.7 cells, rat thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87962-1-RR targets USP21 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12316

  Product name: Recombinant human USP21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 216-565 aa of BC090946

  Sequence: NLGNTCFLNAVLQCLSSTRPLRDFCLRRDFRQEVPGGGRAQELTEAFADVIGALWHPDSCEAVNPTRFRAVFQKYVPSFSGYSQQDAQEFLKLLMERLHLEINRRGRRAPPILANGPVPSPPRRGGALLEEPELSDDDRANLMWKRYLEREDSKIVDLFVGQLKSCLKCQACGYRSTTFEVFCDLSLPIPKKGFAGGKVSLRDCFNLFTKEEELESENAPVCDRCRQKTRSTKKLTVQRFPRILVLHLNRFSASRGSIKKSSVGVDFPLQRLSLGDFASDKAGSPVYQLYALCNHSGSVHYGHYTALCRCQTGWHVYNDSRVSPVSENQVASSEGYVLFYQLMQEPPRCL

• Full Name: ubiquitin specific peptidase 21
• Calculated Molecular Weight: 565 aa, 63 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC090946
• Gene Symbol: USP21
• Gene ID (NCBI): 27005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UK80
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP21(Ubiquitin carboxyl-terminal hydrolase 21) is also named as USP23.The USPs belong to a subclass of the protein-deubiquitinating enzyme (DUB) superfamily that are categorized into five subclasses based on their ubiquitin-protease domains in the human genome and have been shown to be involved in a broad range of biological activities(PMID:19910467). USP21 is expressed at different levels in the tissues examined. The highest level of USP21 expression is found in the heart, pancreas, and skeletal muscle. USP21 messages could also be detected in the brain, placenta, liver, and kidney but are very low in the Lung through the northern blot(PMID:10799498).