USP22 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP22 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 55110-1-AP

Synonyms

Deubiquitinating enzyme 22, KIAA1063, Ubiquitin thiolesterase 22, USP22, USP3L

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Validation Data Gallery

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  WB analysis of A549 using 55110-1-AP

  WB result of USP22 antibody (55110-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP22 transfected A549 cells.

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  WB analysis using 55110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55110-1-AP (USP22 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 55110-1-AP

  IP result of anti-USP22 (IP:55110-1-AP, 4ug; Detection:55110-1-AP 1:500) with HepG2 cells lysate 4000 ug.

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  IHC staining of human lung cancer using 55110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55110-1-AP (USP22 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55110-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55110-1-AP (USP22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, HepG2 cells
• Positive IP detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

55110-1-AP targets USP22 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 22
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 58-60 kDa
• GenBank Accession Number: NM_015276
• Gene Symbol: USP22
• Gene ID (NCBI): 23326
• RRID: AB_10859943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.

Publications

Species	Application	Title
human	WB	Sci Adv; PMID: 29732405; Authors - Qingxiang Liu
mouse	WB	Nat Commun; ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.; Authors - Francesca Bufalieri
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
rat	WB,IF	Eur J Pharmacol; USP22 aggravated diabetic renal tubulointerstitial fibrosis progression through deubiquitinating and stabilizing Snail1; Authors - Xilin Zhao ; KD Validated
human	WB,CoIP	Oncol Rep; Targeting USP22 with miR‑30‑5p to inhibit the hypoxia‑induced expression of PD‑L1 in lung adenocarcinoma cells.; Authors - Xiaoyang Hua
rat	WB	Endocrinology; AGEs-RAGE system down-regulates Sirt1 through the ubiquitin-proteasome pathway to promote FN and TGF-β1 expression in male rat glomerular mesangial cells; Authors - Kai-Peng Huang ; KD Validated