Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells |
| Positive IP detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55110-1-AP targets USP22 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | ubiquitin specific peptidase 22 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 58-60 kDa |
| GenBank Accession Number | NM_015276 |
| Gene Symbol | USP22 |
| Gene ID (NCBI) | 23326 |
| RRID | AB_10859943 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP22, also named as KIAA1063 and USP3L, belongs to the peptidase C19 family and UBP8 subfamily. It is histone deubiquitinating component of the transcription regulatory histone acetylation (HAT) complex SAGA. USP22 catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a coactivator. It is recruited to specific gene promoters by activators such as MYC, where it is required for transcription. USP22 is required for nuclear receptor-mediated transactivation and cell cycle progression. USP22 has 2 isoforms with molecular mass of 58kDa and 60 kDa. The antibody is specific to USP22.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP22 antibody 55110-1-AP | Download protocol |
| IP protocol for USP22 antibody 55110-1-AP | Download protocol |
| WB protocol for USP22 antibody 55110-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
USP22 antibody (Cat# 55110-1-AP) has accumulated 23 citations across journals including Nature Communications, Science Translational Medicine, Journal of Clinical Investigation, Science Advances, Cell Death & Disease, Molecular Oncology, and others. Research fields span oncology (hepatocellular carcinoma, gastric cancer, ovarian cancer, lung adenocarcinoma, pancreatic cancer, AML), fibrosis (cardiac and renal), inflammation, immune signaling, antiviral responses, diabetic complications, and neurology.
| Species | Application | Title |
|---|---|---|
Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
Sci Transl Med USP5 inhibition enables potential therapy for t(8;21) AML through ubiquitin-mediated AML1-ETO degradation in patient-derived xenografts | ||
J Clin Invest SIRT2-mediated deacetylation activates USP22 catalytic function for PD-L1 protein stabilization and tumor immune escape. | ||
Cell Death Dis USP22 upregulates ZEB1-mediated VEGFA transcription in hepatocellular carcinoma
| ||
Int J Biol Sci O-GlcNAcylated RALY Contributes to Hepatocellular Carcinoma Cells Proliferation by Regulating USP22 mRNA Nuclear Export |
