USP24 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP24 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 13126-1-AP

Synonyms

Ubiquitin thiolesterase 24, Ubiquitin thioesterase 24, Ubiquitin carboxyl-terminal hydrolase 24, KIAA1057, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis of HepG2 using 13126-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13126-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 13126-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 13126-1-AP (USP24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13126-1-AP

  IP result of anti-USP24 (IP:13126-1-AP, 4ug; Detection:13126-1-AP 1:3000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 13126-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13126-1-AP (USP24 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13126-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13126-1-AP (USP24 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse lung tissue, rat lung tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 14 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Product Information

13126-1-AP targets USP24 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP24 fusion protein Ag3779
• Full Name: ubiquitin specific peptidase 24
• Calculated Molecular Weight: 294 kDa
• Observed Molecular Weight: 280-294 kDa
• GenBank Accession Number: BC029660
• Gene Symbol: USP24
• Gene ID (NCBI): 23358
• RRID: AB_2212761
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer; Authors - Zeguo Sun
human	WB,IHC,IF	Nat Commun; USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.; Authors - Yi-Chang Wang ; KD Validated
mouse	WB	Nat Commun; A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.; Authors - Jung-Min Kim ; KD Validated
mouse		Nat Commun; ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP.; Authors - Francesca Bufalieri
human	IP, WB	Cell Death Differ; USP24 promotes drug resistance during cancer therapy.; Authors - Shao-An Wang
human	WB	EMBO Mol Med; USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma.; Authors - Katharina Engel