Tested Applications
| Positive WB detected in | HepG2 cells, mouse lung tissue, rat lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human lymphoma tissue, rat stomach tissue, human placenta tissue, rat pancreas tissue, mouse liver tissue, mouse stomach tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13126-1-AP targets USP24 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3779 Product name: Recombinant human USP24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2312-2620 aa of BC029660 Sequence: MISFLLGASRQNNQIRRWSSAQAREFGNLHNTVALLVLHSDVSSQRNVAPGIFKQRPPISIAPSSPLLPLHEEVEALLFMSEGKPYLLEVMFALRELTGSLLALIEMVVYCCFCNEHFSFTMLHFIKNQLETAPPHELKNTFQLLHEILVIEDPIQVERVKFVFETENGLLALMHHSNHVDSSRCYQCVKFLVTLAQKCPAAKEYFKENSHHWSWAVQWLQKKMSEHYWTPQSNVSNETSTGKTFQRTISAQDTLAYATALLNEKEQSGSSNGSESSPANENGDRHLQQGSESPMMIGELRSDLDDVDP Predict reactive species |
| Full Name | ubiquitin specific peptidase 24 |
| Calculated Molecular Weight | 294 kDa |
| Observed Molecular Weight | 280-294 kDa |
| GenBank Accession Number | BC029660 |
| Gene Symbol | USP24 |
| Gene ID (NCBI) | 23358 |
| RRID | AB_2212761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPU5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP24 antibody 13126-1-AP | Download protocol |
| IHC protocol for USP24 antibody 13126-1-AP | Download protocol |
| IP protocol for USP24 antibody 13126-1-AP | Download protocol |
| WB protocol for USP24 antibody 13126-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USP24 antibody (Cat# 13126-1-AP) has 25 citations published across 20 journals, including Nature Communications, Molecular Cancer, Cell Death & Differentiation, Science Advances, Autophagy, and Oncogene. Associated research fields encompass oncology (lung, breast, bladder, liver, esophageal cancers), autophagy regulation, ferroptosis, bone biology, neurodegeneration, viral infection, drug resistance, and metabolic/cardiac disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Aberrant Notch-signaling promotes tumor angiogenesis in esophageal squamous-cell carcinoma | ||
Mol Cancer LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer | ||
Autophagy The PARK10 gene USP24 is a negative regulator of autophagy and ULK1 protein stability.
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Nat Commun ERAP1 promotes Hedgehog-dependent tumorigenesis by controlling USP47-mediated degradation of βTrCP. | ||
Nat Commun A RUNX2 stabilization pathway mediates physiologic and pathologic bone formation.
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Nat Commun USP24 induces IL-6 in tumor-associated microenvironment by stabilizing p300 and β-TrCP and promotes cancer malignancy.
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