USP25 Polyclonal antibody

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USP25 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12199-1-AP Copied!

Publications (15)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 25, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 25, Ubiquitin thioesterase 25, Ubiquitin thiolesterase 25

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  WB analysis using 12199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 12199-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse cerebellum using 12199-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12199-1-AP (USP25 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of RAW 264.7 using 12199-1-AP

  IP result of anti-USP25 (IP:12199-1-AP, 4ug; Detection:12199-1-AP 1:300) with RAW 264.7 cells lysate 1600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 12199-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 12199-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12199-1-AP (USP25 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A-673 cells, BxPC-3 cells, J774A.1 cells, mouse cerebellum tissue, HEK-293 cells, K-562 cells, RAW 264.7 cells
• Positive IP detected in: RAW 264.7 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12199-1-AP targets USP25 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2837

  Product name: Recombinant human USP25 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 171-451 aa of BC015930

  Sequence: LKNVGNTCWFSAVIQSLFNLLEFRRLVLNYKPPSNAQDLPRNQKEHRNLPFMRELRYLFALLVGTKRKYVDPSRAVEILKDAFKSNDSQQEWHQDYRKFRETTMYLIIGLENFQRESYIDSLLFLICAYQNNKELLSKGLYRGHDEELISHYRRECLLKLNEQAAELFESGEDREVNNGLIIMNEFIVPFLPLLLVDEMEEKDILAVEDMRNRWCSYLGQEMEPHLQEKLTDFLPKLLDCSMEIKSFHEPPKLPSYSTHELCERFARIMLSLSRTPADGR

• Full Name: ubiquitin specific peptidase 25
• Calculated Molecular Weight: 125 kDa
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC015930
• Gene Symbol: USP25
• Gene ID (NCBI): 29761
• RRID: AB_2212771
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHP3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin carboxyl-terminal hydrolase 25, encoded by USP25 gene, has three isoforms by alternative splicing. Isoform USP25a is found in most adult and fetal tissues; Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific, and is upregulated during myogenesis, therefore, may have a role in the regulation of muscular differentiation and function. USP25, as deubiquitinating enzyme that hydrolyzes ubiquitin moieties conjugated to substrates, functions to process newly synthesized Ubiquitin, to recycle ubiquitin molecules or to edit polyubiquitin chains and prevents proteasomal degradation of substrates.

Publications

What published studies show

The USP25 Polyclonal Antibody (Cat# 12199-1-AP) has 15 citations published in journals including Molecular Cell, Nucleic Acids Research, Advanced Science, Cell Death & Disease, Biochemical Pharmacology, EMBO Reports, Scientific Reports, Journal of Biological Chemistry, Journal of Cellular Physiology, Journal of Cell Science, and Renal Failure. Research fields span DNA damage repair, ubiquitin signaling, cancer (lung, ovarian, liver, pancreatic), ferroptosis, kidney disease, osteoporosis, recurrent miscarriage, adipocyte biology, and nephritis.

Automatically generated from the 15 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; An Interaction Landscape of Ubiquitin Signaling.; Authors - Xiaofei Zhang
human	WB	Nucleic Acids Res; The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage; Authors - Chao Jin
		Adv Sci (Weinh); PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer.; Authors - Yunshang Chen
		Adv Sci (Weinh); USP25 Elevates SHLD2-Mediated DNA Double-Strand Break Repair and Regulates Chemoresponse in Cancer; Authors - Yunhui Li
		Cell Death Dis; TRMT1-mediated tRNA m(2)(2)G modification drives Osimertinib resistance via the ATXN3/USP25 axis in lung adenocarcinoma.; Authors - Jiaqi Li
human	WB	Cell Death Dis; Suppression of USP7 induces BCR-ABL degradation and chronic myelogenous leukemia cell apoptosis.; Authors - Shuoyi Jiang