USP3 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Reviews (1)

Cat no : 12490-1-AP

Synonyms

Deubiquitinating enzyme 3, SIH003, ubiquitin specific peptidase 3, Ubiquitin thiolesterase 3, UBP, USP3

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Validation Data Gallery

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  WB analysis using 12490-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 12490-1-AP

  WB result of USP3 antibody (12490-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP3 transfected K-562 cells.

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  WB analysis of RAW264.7 using 12490-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12490-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12490-1-AP (USP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 12490-1-AP

  IP result of anti-USP3 (IP:12490-1-AP, 4ug; Detection:12490-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IF Staining of HeLa using 12490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 12490-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12490-1-AP (USP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 12490-1-AP

  Immunofluorescent analysis of MCF-7 cells using 12490-1-AP (USP3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, RAW264.7, HepG2 cells, Jurkat cells
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 3 publications below

Product Information

12490-1-AP targets USP3 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP3 fusion protein Ag3085
• Full Name: ubiquitin specific peptidase 3
• Calculated Molecular Weight: 520 aa, 59 kDa
• Observed Molecular Weight: 53-59 kDa
• GenBank Accession Number: BC018113
• Gene Symbol: USP3
• Gene ID (NCBI): 9960
• RRID: AB_10639042
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB,IP	Cell Death Differ; N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors; Authors - Zhishuang Gao
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh
human	WB	BMC Med; Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis; Authors - Zhijian Zheng
rat	WB	Cell Rep; Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex; Authors - Kellen D Winden
human	WB	BMC Cancer; Protein deubiquitylase USP3 stabilizes Aurora A to promote proliferation and metastasis of esophageal squamous cell carcinoma.; Authors - Ke Shi ; KD Validated
human	WB,IHC,IF,CoIP	Cell Death Dis; USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation.; Authors - Xiaosheng Wu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (05-08-2019)

Worked well

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2c12 mouse myoblast