Tested Applications
| Positive WB detected in | A549 cells, K-562 cells, RAW264.7, HepG2 cells, Jurkat cells |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12490-1-AP targets USP3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3085 Product name: Recombinant human USP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC018113 Sequence: MECPHLSSSVCIAPDSAKFPNGSPSSWCCSVCRSNKSPWVCLTCSSVHCGRYVNGHAKKHYEDAQVPLTNHKKSEKQDKVQHTVCMDCSSYSTYCYRCDDFVVNDTKLGLVQKVREHLQNLENSAFTADRHKKRKLLENSTLNSKLLKVNGSTTAICATGLRNLGNTCFMNAILQSLSNIEQFCCYFKELPAVELRNGKTAGRRTYHTRSQGDNNVSLVEEFRKTLCALWQGSQTAFSPESLFYVVWKIMPNFRGYQQQDAHEFMRYLLDHLHLELQGGFNGVSRSAILQENSTLSASNK Predict reactive species |
| Full Name | ubiquitin specific peptidase 3 |
| Calculated Molecular Weight | 520 aa, 59 kDa |
| Observed Molecular Weight | 53-59 kDa |
| GenBank Accession Number | BC018113 |
| Gene Symbol | USP3 |
| Gene ID (NCBI) | 9960 |
| RRID | AB_10639042 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6I4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP3(Ubiquitin carboxyl-terminal hydrolase 3) belongs to the peptidase C19 family. It may regulate the DNA damage response (DDR) checkpoint through deubiquitination of H2A at DNA damage sites. USP3 is implicated as a novel chromatin modifier in the maintenance of genome integrity. This protein has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP3 antibody 12490-1-AP | Download protocol |
| IHC protocol for USP3 antibody 12490-1-AP | Download protocol |
| IP protocol for USP3 antibody 12490-1-AP | Download protocol |
| WB protocol for USP3 antibody 12490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USP3 Antibody (catalog 12490-1-AP) has 18 citations. They appear in journals including Cell Mol Immunol, Sci Transl Med, Cell Death Differ, Cell Death Dis, Acta Pharmacol Sin, Aging Dis, Chin Med J, BMC Med, EMBO J, Free Radic Biol Med, Cell Rep, EMBO Rep, Biol Direct, BMC Cancer, and Biochem Biophys Res Commun. Research fields span oncology (gastric, prostate, lung, colorectal, esophageal, gallbladder, osteosarcoma, glioma, AML), ubiquitin-mediated deubiquitination, autophagy, inflammasome/innate immunity, and HIV-1 replication.
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol USP3 deubiquitinates and stabilizes the adapter protein ASC to regulate inflammasome activation
| ||
Sci Transl Med USP5 inhibition enables potential therapy for t(8;21) AML through ubiquitin-mediated AML1-ETO degradation in patient-derived xenografts | ||
Cell Death Differ N6-methyladenosine-modified USP13 induces pro-survival autophagy and imatinib resistance via regulating the stabilization of autophagy-related protein 5 in gastrointestinal stromal tumors | ||
Cell Death Dis USP3 promotes osteosarcoma progression via deubiquitinating EPHA2 and activating the PI3K/AKT signaling pathway
| ||
Cell Death Dis USP3 promotes gastric cancer progression and metastasis by deubiquitination-dependent COL9A3/COL6A5 stabilisation.
| ||
Cell Death Dis RHBDL2 drives lipid metabolic reprogramming in osteosarcoma via USP3-mediated deubiquitination of PPT1.
|
Reviews
What customers say
The single review for this product reports a 5-star rating. The antibody was used for Western Blot at a 1:1000 dilution on C2C12 mouse myoblast cells, and the reviewer noted it "worked well." Overall, the feedback is positive, indicating reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (05-08-2019) | Worked well
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