USP32 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP32 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 18838-1-AP

Synonyms

USP10, Ubiquitin-specific-processing protease 32, Ubiquitin carboxyl-terminal hydrolase 32, Renal carcinoma antigen NY-REN-60, NY REN 60

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Validation Data Gallery

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  WB analysis using 18838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18838-1-AP (USP32 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 18838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas using 18838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 18838-1-AP (USP32 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse testis using 18838-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18838-1-AP (USP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells
• Positive IHC detected in: mouse testis tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• CoIP: See 1 publications below

Product Information

18838-1-AP targets USP32 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 32
• Calculated Molecular Weight: 182 kDa
• Observed Molecular Weight: 230 kDa
• GenBank Accession Number: BC094680
• Gene Symbol: USP32
• Gene ID (NCBI): 84669
• RRID: AB_10598326
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP32 belongs to the peptidase C19 family. USP32 catalytic the reaction :Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol.

Publications

Species	Application	Title
human	WB,CoIP	Acta Biochim Biophys Sin (Shanghai); Roles of the hsa_circ_0013880/USP32/Rap1b axis in the proliferation and apoptosis of acute myeloid leukemia cells; Authors - Heyang Zhang ; KD Validated
human	WB	Biochim Biophys Acta Mol Basis Dis; USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis; Authors - Shun Gao