USP33 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP33 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 20445-1-AP

Synonyms

Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1

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Validation Data Gallery

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  WB analysis using 20445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20445-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20445-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20445-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20445-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20445-1-AP (USP33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20445-1-AP

  IP result of anti-USP33 (IP:20445-1-AP, 4ug; Detection:20445-1-AP 1:300) with HEK-293 cells lysate 2400ug.

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  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20445-1-AP (USP33 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20445-1-AP (USP33 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human kidney using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 40x lens).

• 

  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human prostate cancer using 20445-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20445-1-AP (USP33 Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of U2OS using 20445-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 20445-1-AP (USP33 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, mouse brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 15 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Product Information

20445-1-AP targets USP33 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP33 fusion protein Ag14268
• Full Name: ubiquitin specific peptidase 33
• Calculated Molecular Weight: 942 aa, 107 kDa
• Observed Molecular Weight: 103 kDa, 107 kDa
• GenBank Accession Number: BC016663
• Gene Symbol: USP33
• Gene ID (NCBI): 23032
• RRID: AB_10694439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing. This antibody is specific to USP33.

Publications

Species	Application	Title
human	WB,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li
human	IF, WB	J Cell Biol; Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.; Authors - Jacob A Culver
mouse	WB	Diabetes; Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.; Authors - Yuguo Niu
human,mouse	WB,IP	EMBO Rep; High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.; Authors - Yukang Yuan ; KD Validated
human,mouse	WB	Int J Radiat Oncol Biol Phys; USP11 exacerbates radiation induced pneumonitis by activating endothelial cell inflammatory response via OTUD5-STING signaling; Authors - Yiting Tang
human	WB	Cell Death Dis; NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.; Authors - Min Li ; KD Validated