USP34 Polyclonal antibody

USP34 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18827-1-AP

Synonyms

Deubiquitinating enzyme 34, KIAA0570, KIAA0729, Ubiquitin thiolesterase 34, USP34

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Validation Data Gallery

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  IHC staining of human breast cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18827-1-AP (USP34 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 18827-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18827-1-AP (USP34 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18827-1-AP targets USP34 in IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 34
• Calculated Molecular Weight: 404 kDa
• GenBank Accession Number: BC022783
• Gene Symbol: USP34
• Gene ID (NCBI): 9736
• RRID: AB_2213348
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP34, also named as KIAA0570 and KIAA0729, belongs to the peptidase C19 family. USP34 recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. It is involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. The antibody recognize all these 3 isoforms of USP34.