Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24559-1-AP targets USP35 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20002 Product name: Recombinant human USP35 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-342 aa of BC131489 Sequence: MPGLRRRASWRVSIPGSWPSQTRARLVSSTWATHAMSTASFRPYSWRLSSFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSE Predict reactive species |
| Full Name | ubiquitin specific peptidase 35 |
| Calculated Molecular Weight | 1018 aa, 113 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC131489 |
| Gene Symbol | USP35 |
| Gene ID (NCBI) | 57558 |
| RRID | AB_2879608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2H5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP35 antibody 24559-1-AP | Download protocol |
| WB protocol for USP35 antibody 24559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-USP35 antibody (Cat# 24559-1-AP) has 9 total citations. Citations appear in journals including Cell Death Discovery, Free Radical Biology and Medicine, Oncogenesis, Communications Biology, American Journal of Physiology – Cell Physiology, Translational Oncology, Biochemical and Biophysical Research Communications, and Open Life Sciences. Research fields span prostate tumorigenesis, renal ferroptosis, diabetic wound healing, colorectal cancer, breast cancer glycolysis, hepatocellular carcinoma, neuroprotection, and protein stability regulation.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis | ||
Cell Death Discov Deubiquitinase USP35 regulates MDM4 degradation to promote endothelial ferroptosis and renal injury progression. | ||
Free Radic Biol Med Echinacoside Improves Antioxidant Responses and Angiogenesis Through Parkin-MFN2-Mediated Mitophagy to Promote Diabetic Wound Healing.
| ||
Oncogenesis USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer | ||
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1
| ||
Am J Physiol Cell Physiol USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis |
