USP37 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP37 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 18465-1-AP

Synonyms

Deubiquitinating enzyme 37, KIAA1594, Ubiquitin thiolesterase 37, USP37

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Validation Data Gallery

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  WB analysis using 18465-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18465-1-AP (USP37 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18465-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18465-1-AP (USP37 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 18465-1-AP

  IP result of anti-USP37 (IP:18465-1-AP, 4ug; Detection:18465-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 1010x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 4040x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 18465-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18465-1-AP (USP37 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse spleen tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 2 publications below

Product Information

18465-1-AP targets USP37 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP37 fusion protein Ag12513
• Full Name: ubiquitin specific peptidase 37
• Calculated Molecular Weight: 885 aa, 100 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC112901
• Gene Symbol: USP37
• Gene ID (NCBI): 57695
• RRID: AB_10598483
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719).

Publications

Species	Application	Title
human	WB,IF,IHC	Nucleic Acids Res; USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.; Authors - Chenming Wu ; KD Validated
human	WB	Proc Natl Acad Sci U S A; CYLD regulates spindle orientation by stabilizing astral microtubules and promoting dishevelled-NuMA-dynein/dynactin complex formation.; Authors - Yunfan Yang
human	IF	Theranostics; PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.; Authors - Liang Wu ; KD Validated
human	WB	Front Immunol; Specific Deubiquitinating Enzymes Promote Host Restriction Factors Against HIV/SIV Viruses.; Authors - Wenying Gao ; KD Validated
human	WB,IHC,IF	J Exp Clin Cancer Res; PMID: 30482232; Authors - Tao Qin ; KD Validated
human	WB, IP	PLoS One; The HBx Oncoprotein of Hepatitis B Virus Deregulates the Cell Cycle by Promoting the Intracellular Accumulation and Re-Compartmentalization of the Cellular Deubiquitinase USP37.; Authors - Nehul Saxena