Tested Applications
| Positive WB detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, rat brain tissue, rat ovary tissue, mouse adrenal gland tissue, mouse ovary tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18465-1-AP targets USP37 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12513 Product name: Recombinant human USP37 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC112901 Sequence: MLTLQDNSFLSIDKVPSKDAEEMRLFLDAVHQNRLPAAMKPSQGSGSFGAILGSRTSQKETSRQLSYSDNQASAKRGSLETKDDIPFRKVLGNPGRGSIKTVAGSGIARTIPSLTSTSTPLRSGLLENRTEKRKRMISTGSELNEDYPKENDSSSNNKAMTDPSRKYLTSSREKQLSLKQSEENRTSGLLPLQSSSFYGSRAGSKEHSSGGTNLDRTNVSSQTPSAKRSLGFLPQPVPLSVKKLRCNQDYTGWNKPRVPLSSHQQQQLQGFSNLGNTCYMNAILQSLFSLQSFANDLLKQGIPWKKIPLNALIRRFAHLLVKKDICNSETKKDLLKKVKNAISATAERFSG Predict reactive species |
| Full Name | ubiquitin specific peptidase 37 |
| Calculated Molecular Weight | 885 aa, 100 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC112901 |
| Gene Symbol | USP37 |
| Gene ID (NCBI) | 57695 |
| RRID | AB_10598483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86T82 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP37 (ubiquitin specific peptidase 37), is a member of ubiquitin-specific processing proteases family. Human USP37, localized mainly in the cytoplasm, is composed of 979 amino acids harboring three ubiquitin-interacting motifs between the Cys box and His box of the primary sequence (PMID: 27045719, 24324262). High levels of USP37 gene expression in lung cancer promoted cell viability as well as the Warburg effect via deubiquitination and stabilization of pluripotent factor c-Myc protein (PMID: 25284584). Moreover, USP37 expression was upregulated in breast cancer tissues compared with surrounding tissues and its overexpression was significantly correlated with increased rates of mortality. USP37 may represent a novel molecular target for breast cancer treatment (PMID: 30482232, 27045719).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP37 antibody 18465-1-AP | Download protocol |
| IP protocol for USP37 antibody 18465-1-AP | Download protocol |
| WB protocol for USP37 antibody 18465-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
USP37 Polyclonal antibody (Cat# 18465-1-AP) has 17 citations in journals including Nucleic Acids Research, Theranostics, Journal of Experimental & Clinical Cancer Research, Cell Death & Disease, Cancer Letters, PNAS, Frontiers in Immunology, Frontiers in Pharmacology, Frontiers in Cell and Developmental Biology, Cancer Science, Journal of Virology, and Oncogene. Research fields span cancer biology (breast, lung, gastric, prostate, pancreatic), DNA damage response, neurogenesis, virology (HIV/SIV, HBV), and deubiquitination mechanisms.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res USP37 regulates DNA damage response through stabilizing and deubiquitinating BLM.
| ||
Nucleic Acids Res Topoisomerase IIA in adult NSCs regulates SVZ neurogenesis by transcriptional activation of Usp37 | ||
Theranostics PLAGL2 promotes the proliferation and migration of gastric cancer cells via USP37-mediated deubiquitination of Snail1.
| ||
Cell Death Dis USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer | ||
