Tested Applications
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17767-1-AP targets USP38 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12172 Product name: Recombinant human USP38 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 693-1042 aa of BC068975 Sequence: VPNESNKILVNKDVPQKPGGETTPSVTDLLNYFLAPEILTGDNQYYCENCASLQNAEKTMQITEEPEYLILTLLRFSYDQKYHVRRKILDNVSLPLVLELPVKRITSFSSLSESWSVDVDFTDLSENLAKKLKPSGTDEASCTKLVPYLLSSVVVHSGISSESGHYYSYARNITSTDSSYQMYHQSEALALASSQSHLLGRDSPSAVFEQDLENKEMSKEWFLFNDSRVTFTSFQSVQKITSRFPKDTAYVLLYKKQHSTNGLSGNNPTSGLWINGDPPLQKELMDAITKDNKLYLQEQELNARARALQAASASCSFRPNGFDDNDPPGSCGPTGGGGGGGFNTVGRLVF Predict reactive species |
| Full Name | ubiquitin specific peptidase 38 |
| Calculated Molecular Weight | 1042 aa, 117 kDa |
| Observed Molecular Weight | 117 kDa |
| GenBank Accession Number | BC068975 |
| Gene Symbol | USP38 |
| Gene ID (NCBI) | 84640 |
| RRID | AB_2213383 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NB14 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ubiquitin specific peptidase 38 (USP38) is a negative regulator of type I IFN signaling. USP38 can strongly induced the degradation of the active form of TBK1 after viral infection. USP38 may play a critical role in the maintenance of the balance between innate immune responses and tolerance through dynamic ubiquitin editing of TBK1 in response to viral infection and perhaps other infectious diseases or cancer. (PMID:27692986)
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for USP38 antibody 17767-1-AP | Download protocol |
| WB protocol for USP38 antibody 17767-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-USP38 antibody (Cat# 17767-1-AP) has 14 citations published in journals including Cell Death & Disease, J Exp Med, Mol Med, Oncogenesis, Int Immunopharmacol, J Biol Chem, J Mol Cell Cardiol, Int J Biochem Cell Biol, Am J Cancer Res, Gene, and Cell Mol Life Sci. Research fields span ferroptosis, cancer (breast, colorectal, gastric, esophageal), cardiovascular disease, asthma, sepsis, diabetic nephropathy, and stroke.
| Species | Application | Title |
|---|---|---|
Cell Death Dis The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein | ||
J Exp Med USP38 critically promotes asthmatic pathogenesis by stabilizing JunB protein.
| ||
Mol Med USP38 exacerbates pressure overload-induced left ventricular electrical remodeling | ||
Oncogenesis USP38 regulates the stemness and chemoresistance of human colorectal cancer via regulation of HDAC3.
| ||
Int Immunopharmacol Inhibiting ubiquitin-specific protease 38 safeguards against diabetic nephropathy by limiting tubular epithelial cell ferroptotic death through the suppression of IREB2-mediated iron overload. | ||
Int Immunopharmacol USP38 exacerbates myocardial injury and malignant ventricular arrhythmias after ischemia/reperfusion by promoting ferroptosis through the P53/SLC7A11 pathway |
