USP44 Polyclonal antibody

USP44 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15521-1-AP

Synonyms

Deubiquitinating enzyme 44, Ubiquitin thiolesterase 44, USP44

---

Validation Data Gallery

• 

  WB analysis of human placenta using 15521-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15521-1-AP (USP44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15521-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15521-1-AP (USP44 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: human placenta tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15521-1-AP targets USP44 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP44 fusion protein Ag7888
• Full Name: ubiquitin specific peptidase 44
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC030704
• Gene Symbol: USP44
• Gene ID (NCBI): 84101
• RRID: AB_2213688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP44(Ubiquitin carboxyl-terminal hydrolase 44) belongs to a large family of cysteine proteases that function as deubiquitinating enzymes. It has a ZnF-UBP (zinc-finger ubiquitin-specific protease) domain and conserved cysteine, histidine and asparagine/aspartic acid residues characteristic of deubiquitinating enzymes. And USP44 can cleave ubiquitin from ubiquitinated substrates both in vitro and in vivo. The expression of endogenous mouse or human USP44 is very low. A localization study of USP44 showed its predominant expression in the nucleus(PMID:20402667).

Publications

Species	Application	Title
		J Clin Invest; USP44 regulates centrosome positioning to prevent aneuploidy and suppress tumorigenesis.; Authors - Zhang Ying Y
human		Nucleic Acids Res; USP11 acts as a histone deubiquitinase functioning in chromatin reorganization during DNA repair.; Authors - Xia Ting