USP46 Polyclonal antibody

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  WB analysis of human kidney using 13502-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 13502-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 13502-1-AP (USP46 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 13502-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 13502-1-AP (USP46 Antibody) at dilution of 1:200 (under 40x lens).

• Featured Product
• KD/KO Validated

USP46 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13502-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 46, EC:3.4.19.12, Ubiquitin carboxyl-terminal hydrolase 46, Ubiquitin thioesterase 46, Ubiquitin thiolesterase 46

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Tested Applications

• Positive WB detected in: human kidney tissue, mouse pancreas tissue
• Positive IHC detected in: human renal cell carcinoma tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

13502-1-AP targets USP46 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4330

  Product name: Recombinant human USP46 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-366 aa of BC037574

  Sequence: MTVRNIASICNMGTNASALEKDIGPEQFPINEHYFGLVNFGNTCYCNSVLQALYFCRPFRENVLAYKAQQKKKENLLTCLADLFHSIATQKKKVGVIPPKKFISRLRKENDLFDNYMQQDAHEFLNYLLNTIADILQEEKKQEKQNGKLKNGNMNEPAENNKPELTWVHEIFQGTLTNETRCLNCETVSSKDEDFLDLSVDVEQNTSITHCLRDFSNTETLCSEQKYYCETCCSKQEAQKRMRVKKLPMILALHLKRFKYMEQLHRYTKLSYRVVFPLELRLFNTSSDAVNLDRMYDLVAVVVHCGSGPNRGHYITIVKSHGFWLLFDDDIVEKIDAQAIEEFYGLTSDISKNSESGYILFYQSRE

• Full Name: ubiquitin specific peptidase 46
• Calculated Molecular Weight: 366 aa, 42 kDa
• Observed Molecular Weight: 38-42 kDa
• GenBank Accession Number: BC037574
• Gene Symbol: USP46
• Gene ID (NCBI): 64854
• RRID: AB_10642951
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P62068
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IP	Nat Commun; The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling; Authors - Victoria H Ng
mouse	WB	J Exp Med; USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.; Authors - Zhongxia Yu ; KD Validated
human	WB,IP,IF,CoIP	Biochim Biophys Acta Mol Basis Dis; USP46 enhances tamoxifen resistance in breast cancer cells by stabilizing PTBP1 to facilitate glycolysis; Authors - Shun Gao
mouse	WB,IF	Mol Neurobiol; Ceftriaxone Modulates Ubiquitination of α-Amino-3-Hydroxy-5-Methyl-4-Isoxazole Propionic Acid Receptors to Improve Long-Term Potentiation Impairment Induced by Exogenous β-Amyloid in a Glutamate Transporter-1 Dependent Manner; Authors - Rui Jiang
human	WB,IHC,IF,CoIP	Exp Cell Res; Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma.; Authors - Maoqing Tian ; KD Validated
human	WB	FEBS Open Bio; Proximity-dependent biotinylation reveals an interaction between ubiquitin-specific peptidase 46 and centrosome-related proteins; Authors - Kazuma Yoshioka