USP48 Polyclonal antibody

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  WB analysis of PC-3 using 12076-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12076-1-AP (USP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12076-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12076-1-AP (USP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12076-1-AP

  IP result of anti-USP48 (IP:12076-1-AP, 4ug; Detection:12076-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of human pancreas cancer using 12076-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12076-1-AP (USP48 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12076-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12076-1-AP (USP48 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP48 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12076-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

Deubiquitinating enzyme 48, EC:3.4.19.12, RAP1GA1, Ubiquitin carboxyl-terminal hydrolase 48, Ubiquitin thiolesterase 48

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

12076-1-AP targets USP48 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2749

  Product name: Recombinant human USP48 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 782-1035 aa of BC013567

  Sequence: MFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH

• Full Name: ubiquitin specific peptidase 48
• Calculated Molecular Weight: 1035 aa, 119 kDa
• Observed Molecular Weight: 119 kDa
• GenBank Accession Number: BC013567
• Gene Symbol: USP48
• Gene ID (NCBI): 84196
• RRID: AB_2213840
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86UV5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,IHC,IF	Acta Pharm Sin B; Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis; Authors - Can Cheng
	WB	Cancer Res; YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis.; Authors - Shuai Ye
human	IHC	Rheumatology (Oxford); Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β.; Authors - Christine Galant
mouse	IP	Eur J Pain; USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice; Authors - Jiacheng Yu
mouse	WB	FASEB J; Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination; Authors - Ping Wang
human	CoIP	Sci Rep; RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31; Authors - Wei Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachel (Verified Customer) (09-10-2020)

Antibody detected band of the expected height by western blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T