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Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
12076-1-AP targets USP48 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP48 fusion protein Ag2749 Predict reactive species |
| Full Name | ubiquitin specific peptidase 48 |
| Calculated Molecular Weight | 1035 aa, 119 kDa |
| Observed Molecular Weight | 119 kDa |
| GenBank Accession Number | BC013567 |
| Gene Symbol | USP48 |
| Gene ID (NCBI) | 84196 |
| RRID | AB_2213840 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP48 antibody 12076-1-AP | Download protocol |
| IHC protocol for USP48 antibody 12076-1-AP | Download protocol |
| IP protocol for USP48 antibody 12076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Cancer Res YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis. | ||
Rheumatology (Oxford) Overexpression of ubiquitin-specific peptidase 15 in systemic sclerosis fibroblasts increases response to transforming growth factor β. | ||
Eur J Pain USP48 alleviates bone cancer pain and regulates MrgC stabilization in spinal cord neurons of male mice | ||
FASEB J Candesartan upregulates angiotensin-converting enzyme 2 in kidneys of male animals by decreased ubiquitination | ||
Sci Rep RUNX1 regulates MCM2/CDC20 to promote COAD progression modified by deubiquitination of USP31 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Rachel (Verified Customer) (09-10-2020) | Antibody detected band of the expected height by western blot.
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