Tested Applications
| Positive WB detected in | PC-3 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12076-1-AP targets USP48 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2749 Product name: Recombinant human USP48 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 782-1035 aa of BC013567 Sequence: MFTFASMTKEDSKLIALIWPSEWQMIQKLFVVDHVIKITRIEVGDVNPSETQYISEPKLCPECREGLLCQQQRDLREYTQATIYVHKVVDNKKVMKDSAPELNVSSSETEEDKEEAKPDGEKDPDFNQSNGGTKRQKISHQNYIAYQKQVIRRSMRHRKVRGEKALLVSANQTLKELKIQIMHAFSVAPFDQNLSIDGKILSDDCATLGTLGVIPESVILLKADEPIADYAAMDDVMQVCMPEEGFKGTGLLGH Predict reactive species |
| Full Name | ubiquitin specific peptidase 48 |
| Calculated Molecular Weight | 1035 aa, 119 kDa |
| Observed Molecular Weight | 119 kDa |
| GenBank Accession Number | BC013567 |
| Gene Symbol | USP48 |
| Gene ID (NCBI) | 84196 |
| RRID | AB_2213840 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86UV5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP48 (Ubiquitin Specific Peptidase 48) is a deubiquitinating enzyme and a member of the Ubiquitin Specific Protease (USP) family. This protein specifically recognizes and cleaves ubiquitin chains on substrate proteins, reversing the ubiquitination process, thereby regulating the stability, activity, and subcellular localization of target proteins. USP48 plays a key role in various cellular processes, including DNA damage repair, cell cycle regulation, and gene transcription. Studies have shown that USP48 is closely related to pathological processes such as cancer and neurodegenerative diseases, for example, by stabilizing transcription factors or signaling pathway proteins to influence tumor development.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP48 antibody 12076-1-AP | Download protocol |
| IP protocol for USP48 antibody 12076-1-AP | Download protocol |
| WB protocol for USP48 antibody 12076-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USP48 antibody (Cat# 12076-1-AP) has 14 citations published in journals including Cancer Research, Science Translational Medicine, Acta Pharmaceutica Sinica B, Cell Death & Disease, FASEB Journal, International Immunopharmacology, and Molecular Carcinogenesis. Research fields span oncology (sarcoma, AML, colorectal, breast, and gastric cancer), ferroptosis, pyroptosis, sepsis-induced lung injury, systemic sclerosis, and deubiquitination-mediated protein regulation.
| Species | Application | Title |
|---|---|---|
Cancer Res YAP1-mediated suppression of USP31 enhances NF-κB activity to promote sarcomagenesis. | ||
Sci Transl Med USP5 inhibition enables potential therapy for t(8;21) AML through ubiquitin-mediated AML1-ETO degradation in patient-derived xenografts | ||
Acta Pharm Sin B Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis | ||
Cell Death Dis The deubiquitinase USP24 suppresses ferroptosis in triple-negative breast cancer by stabilizing DHODH protein | ||
Cancer Biol Ther Caspase 3-specific cleavage of ubiquitin-specific peptidase 48 enhances drug-induced apoptosis in AML | ||
Int Immunopharmacol USP48 promotes NLRP3-dependent pyroptosis of alveolar macrophages to exacerbate sepsis-induced acute lung injury.
|
Reviews
What customers say
One review was submitted for this product. The reviewer used the antibody for Western Blot on HEK293T cells at a 1/1000 dilution and reported that it detected a band of the expected size. The overall rating was 3 out of 5, suggesting acceptable but not exceptional performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Rachel (Verified Customer) (09-10-2020) | Antibody detected band of the expected height by western blot.
|
