USP49 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP49 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 18066-1-AP

Synonyms

Ubiquitin-specific-processing protease 49, Ubiquitin thiolesterase 49, Ubiquitin thioesterase 49, Ubiquitin carboxyl-terminal hydrolase 49, EC:3.4.19.12

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 18066-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 18066-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18066-1-AP (USP49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 18066-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18066-1-AP (USP49 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human colon cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 11 publications below
• WB: See 15 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Product Information

18066-1-AP targets USP49 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP49 fusion protein Ag12697
• Full Name: ubiquitin specific peptidase 49
• Calculated Molecular Weight: 73 kDa, 79 kDa
• Observed Molecular Weight: 79-85 kDa
• GenBank Accession Number: BC014176
• Gene Symbol: USP49
• Gene ID (NCBI): 25862
• RRID: AB_10643992
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USPs are cysteine proteases that vary greatly in size and structural complexity. Outside of the core catalytic domain, USPs exhibit N-terminal and C-terminal extensions that have been proposed to play roles in determining cellular localization and substrate specificity of these enzymes(PMID:14715245). USP49(Ubiquitin carboxyl-terminal hydrolase 49) belongs to this family. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Cell Death Differ; Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis.; Authors - Kamini Kaushal ; KO Validated
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
human	WB	Theranostics; USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2; Authors - Arun Pandian Chandrasekaran ; KD Validated
human	WB	Elife; USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication.; Authors - Ting Pan ; KD Validated
human	WB,CoIP	Carcinogenesis; USP49 promotes adenocarcinoma of the esophagogastric junction malignant progression via activating SHCBP1-β-catenin-GPX4 axis; Authors - Yun Ding ; KD Validated
human	WB	J Cell Mol Med; CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma.; Authors - Jing Chen