USP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 10473-1-AP

Synonyms

Ubiquitin carboxyl-terminal hydrolase 5, ISOT 1, ISOT, Isopeptidase T, EC:3.4.19.12

---

Validation Data Gallery

• 

  WB analysis of A549 using 10473-1-AP

  WB result of USP5 antibody (10473-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells.

• 

  WB analysis using 10473-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10473-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 10473-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 10473-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 10473-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10473-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U2OS using 10473-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 10473-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 10473-1-AP (USP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10473-1-AP

  IP result of anti-USP5 (IP:10473-1-AP, 4ug; Detection:10473-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human heart using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 10473-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 10473-1-AP (USP5 antibody) at dilution of 1:200 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A549 cells, mouse brain tissue, human heart tissue, human colon tissue, U2OS cells, DU 145 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human heart tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 36 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• IP: See 5 publications below
• ELISA: See 2 publications below
• CoIP: See 3 publications below

Product Information

10473-1-AP targets USP5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP5 fusion protein Ag0770
• Full Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC004889
• Gene Symbol: USP5
• Gene ID (NCBI): 8078
• RRID: AB_2272754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 has 2 isoforms with the molecular mass of 96 kDa and 93 kDa.

Publications

Species	Application	Title
mouse	WB	Nat Commun; STREAMING-tag system reveals spatiotemporal relationships between transcriptional regulatory factors and transcriptional activity; Authors - Hiroaki Ohishi
		Blood; The ubiquitin ligase HERC4 mediates c-Maf ubiquitination and delays the growth of multiple myeloma xenografts in nude mice.; Authors - Zubin Zhang
human	WB	Theranostics; Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor.; Authors - Xin Xu ; KD Validated
human	WB	Clin Transl Med; MAFG-AS1 promotes tumor progression via regulation of the HuR/PTBP1 axis in bladder urothelial carcinoma.; Authors - Mengqing Xiao ; KD Validated
human	WB	Acta Pharmacol Sin; Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.; Authors - Xue-Han Chen
rat	WB,IP	Sci Signal; Selective inhibition of CaV3.2 channels reverses hyperexcitability of peripheral nociceptors and alleviates postsurgical pain.; Authors - Sonja L Joksimovic