USP5 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66213-1-Ig (same clone as 66213-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66213-1-Ig (same clone as 66213-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  WB result of USP5 antibody (66213-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP5 transfected A549 cells. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66213-1-Ig (same clone as 66213-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66213-1-Ig (same clone as 66213-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66213-1-Ig (USP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66213-1-Ig (same clone as 66213-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP5 antibody (66213-1-Ig, Clone: 3F12C10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66213-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51440-1

  Cytometric bead array sample test of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS.

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  Cytometric bead array standard curve of MP51440-1

  Cytometric bead array standard curve of MP51440-1, USP5 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66213-1-PBS. Detection antibody: 66213-2-PBS. Standard:Ag7575. Range: 1.563-200 ng/mL.

• Featured Product
• KD/KO Validated

USP5 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66213-1-PBS

Clone No.3F12C10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

3F12C10, Deubiquitinating enzyme 5, EC:3.4.19.12, Isopeptidase T, ISOT

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Product Information

66213-1-PBS targets USP5 as part of a matched antibody pair:

MP51440-1: 66213-1-PBS capture and 66213-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7575

  Product name: Recombinant human USP5 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC005139

  Sequence: MAELSEEALLSVLPTIRVPKAGDRVHKDECAFSFDTPESEGGLYICMNTFLGFGKQYVERHFNKTGQRVYLHLRRTRRPKEEDPATGTGDPPRKKPTRLAIGVEGGFDLSEEKFELDEDVKIVILPDYLEIARDGLGGLPDIVRDRVTSAVEALLSADSASRKQEVQAWDGEVRQVSKHAFSLKQLDNPARIPPCGWKCSKCDMRENLWLNLTDGSILCGRRYFDGSGGNNHAVEHYRETGYPLAVKLGTITPDGADVYSYDEDDMVLDPSLAEHLSHFGIDMLKMQKTDKTMTELEIDMNQRIGEWELIQESGVPLKPLFGPGYTGIRNLGNSCYLNSVVQVLFSIPDF

• Full Name: ubiquitin specific peptidase 5 (isopeptidase T)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC005139
• Gene Symbol: USP5
• Gene ID (NCBI): 8078
• RRID: AB_2881604
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P45974
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.