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USP50 Polyclonal antibody, PBS Only (20374-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of mouse brain using 20374-1-AP (same clone as 20374-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
WB analysis of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 20374-1-AP (USP50 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of human testis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded human testis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of human hepatocirrhosis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20374-1-AP (USP50 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 20374-1-AP (same clone as 20374-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20374-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 20374-1-AP (same clone as 20374-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 20374-1-AP (USP50 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20374-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

USP50 Polyclonal antibody, PBS Only

Name: USP50 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 20374-1-PBS

USP50 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 20374-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

20374-1-PBS targets USP50 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	ubiquitin specific peptidase 50
Calculated Molecular Weight	39 kDa
Observed Molecular Weight	40 kDa
GenBank Accession Number	NM_203494
Gene Symbol	USP50
Gene ID (NCBI)	373509
RRID	AB_10973680
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q70EL3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function through targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.