USP50 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP50 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24817-1-AP

Synonyms

USP50

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Validation Data Gallery

• 

  WB analysis of mouse testis using 24817-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24817-1-AP (USP50 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 24817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24817-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24817-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 24817-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 24817-1-AP (USP50 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IHC detected in: human hepatocirrhosis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

24817-1-AP targets USP50 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP50 fusion protein Ag19337
• Full Name: ubiquitin specific peptidase 50
• Calculated Molecular Weight: 339 aa, 39 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC146493
• Gene Symbol: USP50
• Gene ID (NCBI): 373509
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitin-specific protease 50 (USP50) belongs to the peptidase C19 family. USP50 has been shown to regulate cell cycle and DNA repair through interaction with the Wee1 protein in transformed human osteosarcoma and human epithelial cell lines. USP50 has also been shown to play a role in inflammasomal function by targeting the adaptor protein (PMID: 29101126). USP50 has 2 isoforms with the molecular mass of 38 and 39 kDa. Sometimes higher molecular weight around 44 kDa can also be observed, which may be a modified variant of USP50.

Publications

Species	Application	Title
human,mouse	WB,IF	Front Immunol; USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis; Authors - Chenye Zhao ; KD Validated