USP9Y Polyclonal antibody

USP9Y Polyclonal Antibody for WB, ELISA

Cat No. 32373-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Deubiquitinating enzyme FAF-Y, DFFRY, EC:3.4.19.12, Fat facets protein-related, Y-linked, Ubiquitin carboxyl-terminal hydrolase 9Y

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Quantity

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WB analysis using 32373-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 32373-1-AP (USP9Y antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32373-1-AP targets USP9Y in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag35809 Product name: Recombinant human USP9Y protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2361-2555 aa of NM_004654 Sequence: KGIPDDRDGLFDTIQRSKNHYQKRAYQCIKCMVALFSSCPVAYQILQGNGDLKRKWTWAVEWLGDELERRPYTGNPQYSYNNWSPPVQSNETANGYFLERSHSARMTLAKACELCPEEEPDDQDAPDEHEPSPSEDAPLYPHSPASQYQQNNHVHGQPYTGPAAHHLNNPQKTGQRTQENYEGNEEVSSPQMKDQ Predict reactive species
Full Name	ubiquitin specific peptidase 9, Y-linked
Calculated Molecular Weight	291kDa
Observed Molecular Weight	250 kDa
GenBank Accession Number	NM_004654
Gene Symbol	USP9Y
Gene ID (NCBI)	8287
RRID	AB_3742583
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity Purification
UNIPROT ID	O00507
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP9Y (Ubiquitin-Specific Peptidase 9 on the Y chromosome) is a gene located in the male-specific region of the Y chromosome and is broadly expressed in male adult tissues. It encodes a functional deubiquitinating enzyme that plays a role in protein turnover (PMID: 39742627).