UTF1 Polyclonal antibody

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• UTF1 Polyclonal antibody (30456-1-AP)

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  WB analysis using 30456-1-AP

  UCMSCs cell lysates were subjected to SDS PAGE followed by western blot with 30456-1-AP (UTF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat liver using 30456-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 30456-1-AP (UTF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

UTF1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30456-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

hUTF 1, hUTF1, UTF 1

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Tested Applications

• Positive WB detected in: UCMSCs cells
• Positive IHC detected in: rat liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30456-1-AP targets UTF1 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31070

  Product name: Recombinant human UTF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 88-265 aa of NM_003577

  Sequence: RRQALPTYRRVSAALAQQQVRRTPAQCRRRYKFLKDKFREAHGQPPGPFDEQIRKLMGLLGDNGRKRPRRRSPGSGRPQRARRPVPNAHAPAPSEPDATPLPTARDRDADPTWTLRFSPSPPKSADASPAPGSPPAPAPTALATCIPEDRAPVRGPGSPPPPPAREDPDSPPGRPEDC

• Full Name: undifferentiated embryonic cell transcription factor 1
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: NM_003577
• Gene Symbol: UTF1
• Gene ID (NCBI): 8433
• RRID: AB_3742344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T230
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UTF1 is a nuclear transcriptional co-regulator expressed in pluripotent stem cells, where it stabilizes self-renewal networks and controls the transition from pluripotency to differentiation. As a chromatin-associated factor, it fine-tunes gene expression programs essential for early embryogenesis and is a key marker in stem cell and cancer biology research.