UTP14A Polyclonal antibody

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  WB analysis of mouse brain using 11474-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11474-1-AP (UTP14A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 11474-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11474-1-AP (UTP14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11474-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11474-1-AP (UTP14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 11474-1-AP

  Immunofluorescent analysis of HepG2 cells, using UTP14A antibody 11474-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of HepG2 using 11474-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UTP14A antibody (11474-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

UTP14A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 11474-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

U3 small nucleolar RNA-associated protein 14 homolog A, SDCCAG16, NY CO 16, KIAA0266, Antigen NY-CO-16

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, PC-3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 2 publications below

Product Information

11474-1-AP targets UTP14A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2027

  Product name: Recombinant human UTP14A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC014987

  Sequence: MTANRLAESLLALSQQEELADLPKDYLLSESEDEGDNDGERKHQKLLEAISSLDGKNRRKLAERSEASLKVSEFNVSSEGSGEKLVLADLLEPVKTSSSLATVKKQLSRVKSKKTVELPLNKEEIERIHREVAFNKTAQVLSKWDPVVLKNRQAEQLVFPLEKEEPAIAPIEHVLSGWKARTPLEQEIFNLLHKNKQPVTDPLLTPVEKASLRAMSLEEAKMRRAELQRARALQSYYEAKARREKKIKSKKYHKVVKKGKAKKALKEFEQLRKVNPAAALEELEKIEKARMMERMSLKHQNSGKWAKSKAIMAKYDLEARQAMQEQLSKNKELTQKLQVASESEEEEGGT

• Full Name: UTP14, U3 small nucleolar ribonucleoprotein, homolog A (yeast)
• Calculated Molecular Weight: 771 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC014987
• Gene Symbol: UTP14A
• Gene ID (NCBI): 10813
• RRID: AB_2272800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BVJ6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Hum Mol Genet; Effects of the Bowen-Conradi syndrome mutation in EMG1 on its nuclear import, stability and nucleolar recruitment.; Authors - Ahmed S Warda ; KD Validated
human	WB,IF	iScience; P16INK4A drives RB1 degradation by UTP14A-catalyzed K810 ubiquitination; Authors - Wenjie Weng
human	IF	RNA Biol; The DExD box ATPase DDX55 is recruited to domain IV of the 28S ribosomal RNA by its C-terminal region.; Authors - Priyanka Choudhury
human	WB	RNA; NSUN6 is a human RNA methyltransferase that catalyzes formation of m5C72 in specific tRNAs.; Authors - Sara Haag
human	WB	J Cancer; U three protein 14a (UTP14A) promotes tumour proliferation and metastasis via the PERK/eIF2a/GRP78 signalling pathway in oesophageal squamous cell carcinoma.; Authors - Kun-Kun Li ; KD Validated
human	WB	Cell Rep; UTP18-mediated p21 mRNA instability drives adenoma-carcinoma progression in colorectal cancer; Authors - Meng Pan