UTP15 Polyclonal antibody

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  WB analysis of MCF-7 using 17416-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17416-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17416-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17416-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17416-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17416-1-AP (UTP15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

UTP15 Polyclonal Antibody for WB, ELISA

Cat No. 17416-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NET21, UTP15

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells, HepG2 cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17416-1-AP targets UTP15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11161

  Product name: Recombinant human UTP15 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC013064

  Sequence: MLKGGQLLVSLKNHHKTVTCLCLSSSGQRLLSGSLDRKVKVYSTTSYKVVHSFDYAASILSLALAHEDETIVVGMTNGILSVKHRKSEAKKESLPRRRRPAYRTFIKGKNYMKQRDDILINRPAKKHLELYDRDLKHFRISKALDRVLDPTCTIKTPEITVSIIKELNRRGVLANALAGRDEKEISHVLNFLIRNLSQPRFAPVLINAAEIIIDIYLPVIGQSPVVDKKFLLLQGLVEKEIDYQRELLETLGMMDMLFATMRRKEGTSVLEHTSDGFPENKKIES

• Full Name: UTP15, U3 small nucleolar ribonucleoprotein, homolog (S. cerevisiae)
• Calculated Molecular Weight: 285aa,32 kDa; 518aa,58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC013064
• Gene Symbol: UTP15
• Gene ID (NCBI): 84135
• RRID: AB_2304317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TED0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UTP15 is a U3 snoRNA-associated protein of the Small Subunit Processome (SSU), essential for 18S rRNA biogenesis. UTP15 has 3 isoforms with MW 56, 58 and 37kDa (refer to UniProt). Catalog#17416-1-AP is a rabbit polyclonal antibody raised against the full-length of human UTP15. It specially recognizes 58 and 37kDa isoforms.