UTP3 Polyclonal antibody

UTP3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18886-1-AP

Synonyms

CRL1, CRLZ1, Disrupter of silencing SAS10, SAS10, UTP3, UTP3 homolog

---

Validation Data Gallery

• 

  WB analysis of A549 using 18886-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 18886-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18886-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18886-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18886-1-AP (UTP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 18886-1-AP

  Immunofluorescent analysis of HepG2 cells, using UTP3 antibody 18886-1-AP at 1:50dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 18886-1-AP

  Immunofluorescent analysis of Hela cells, using UTP3 antibody 18886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, human brain tissue, mouse thymus tissue
• Positive IF/ICC detected in: HepG2 cells, Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18886-1-AP targets UTP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UTP3 fusion protein Ag13454
• Full Name: UTP3, small subunit (SSU) processome component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 479 aa, 55 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC004546
• Gene Symbol: UTP3
• Gene ID (NCBI): 57050
• RRID: AB_10597076
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.