UTX Polyclonal antibody

UTX Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23984-1-AP

Synonyms

Histone demethylase UTX, KDM6A, Lysine specific demethylase 6A, UTX

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Validation Data Gallery

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  WB analysis of COLO 320 using 23984-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23984-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 23984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23984-1-AP targets UTX in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UTX fusion protein Ag20018
• Full Name: ubiquitously transcribed tetratricopeptide repeat, X chromosome
• Calculated Molecular Weight: 1401 aa, 154 kDa
• Observed Molecular Weight: 154 kDa
• GenBank Accession Number: BC093868
• Gene Symbol: UTX
• Gene ID (NCBI): 7403
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM6A, also named UTX, mediates the removal of repressive trimethylation of histone H3, it plays a central role in the regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).