UTX Polyclonal antibody

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• UTX Polyclonal antibody (23984-1-AP)

UTX Polyclonal Antibody for WB, ELISA

Cat No. 23984-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Lysine-specific demethylase 6A, Lysine specific demethylase 6A, KDM6A, Histone demethylase UTX, EC:1.14.11.68

• 

  WB analysis of COLO 320 using 23984-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 23984-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 23984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23984-1-AP (UTX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23984-1-AP targets UTX in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20018

  Product name: Recombinant human UTX protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1059-1401 aa of BC093868

  Sequence: DHSDSESTSSDNSGRRRKGPFKTIKFGTNIDLSDDKKWKLQLHELTKLPAFVRVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFVVPEGYWGVLNDFCEKNNLNFLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWINAGTVHWVQAIGWCNNIAWNVGPLTACQYKLAVERYEWNKLQSVKSIVPMVHLSWNMARNIKVSDPKLFEMIKYCLLRTLKQCQTLREALIAAGKEIIWHGRTKEEPAHYCSICEVEVFDLLFVTNESNSRKTYIVHCQDCARKTSGNLENFVVLEQYKMEDLMQVYDQFTLAPPLPSASS

• Full Name: ubiquitously transcribed tetratricopeptide repeat, X chromosome
• Calculated Molecular Weight: 1401 aa, 154 kDa
• Observed Molecular Weight: 154 kDa
• GenBank Accession Number: BC093868
• Gene Symbol: UTX
• Gene ID (NCBI): 7403
• RRID: AB_2935460
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15550
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KDM6A, also named UTX, mediates the removal of repressive trimethylation of histone H3, it plays a central role in the regulation of posterior development, by regulating HOX gene expression (PubMed:17851529).

Publications

What published studies show

The UTX Polyclonal antibody (Cat# 23984-1-AP) has a total of 2 citations. These publications appear in Advanced Science and European Journal of Medical Research. The research fields encompass tumor biology and cancer metabolism—specifically, one study investigates copper-targeted EZH2 degradation and anti-PD-1 immunotherapy in oral squamous cell carcinoma, while the other explores KDM6A inactivation promoting colorectal cancer progression through enhanced glycolysis. Both citations utilize the antibody for Western blot in human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Adv Sci (Weinh); Targeting Intratumoral Copper Inhibits Tumor Progression via p62-Mediated EZH2 Degradation and Potentiates Anti-PD-1 Immunotherapy in Oral Squamous Cell Carcinoma; Authors - Xiaohu Lin
human	WB	Eur J Med Res; Inactivation of KDM6A promotes the progression of colorectal cancer by enhancing the glycolysis; Authors - Dexiang Zhang