UXT Polyclonal antibody

UXT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11047-1-AP

Synonyms

ART 27, Protein UXT, UXT

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Validation Data Gallery

• 

  WB analysis of HeLa using 11047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11047-1-AP (UXT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11047-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11047-1-AP (UXT antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11047-1-AP

  IP result of anti-UXT (IP:11047-1-AP, 3ug; Detection:11047-1-AP 1:300) with mouse brain tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11047-1-AP targets UXT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UXT fusion protein Ag1513
• Full Name: ubiquitously-expressed transcript
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 18 kDa, 45 kDa
• GenBank Accession Number: BC008890
• Gene Symbol: UXT
• Gene ID (NCBI): 8409
• RRID: AB_2241535
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquitously expressed transcript (UXT) is a gene transcription regulator. It can regulate androgen receptor(AR) transcription by concerting with the coreperssor UR1. It is proposed a potential component of mitochondrial-associated LRPPRC, a multidomain organizer that potentially integrates mitochondria and the microtubular cytoskeleton with chromosome remodeling, for UXT increasing concentrations of UXT contributes to progressive aggregation of mitochondria and cell death potentially through its association with LRPPRC. UXT is possible a nuclear chaperone that promotes formation of the NF-kappa-B enhanceosome and which is essential for its nuclear function. It can suppresses cell transformation and mediate this function by interaction and inhibition of the biological activity of cell proliferation and survival stimulatory factors like MECOM

Publications

Species	Application	Title
mouse	WB	Nature; Long non-coding antisense RNA controls Uchl1 translation through an embedded SINEB2 repeat.; Authors - Carrieri Claudia C