Ubiquilin 1 Polyclonal antibody

Ubiquilin 1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22126-1-AP

Synonyms

UBQLN1, PLIC 1, hPLIC-1, hPLIC1, hPLIC 1

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Validation Data Gallery

• 

  WB analysis of rat brain using 22126-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 22126-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 22126-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 22126-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 22126-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 22126-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 22126-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Ubiquilin 1 antibody (22126-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: rat brain tissue, L02 cells, rat heart tissue, rat liver tissue, Transfected HEK-293 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22126-1-AP targets Ubiquilin 1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ubiquilin 1 fusion protein Ag17530
• Full Name: ubiquilin 1
• Calculated Molecular Weight: 589 aa, 63 kDa
• Observed Molecular Weight: 63-71 kDa
• GenBank Accession Number: BC010066
• Gene Symbol: Ubiquilin 1
• Gene ID (NCBI): 29979
• RRID: AB_2879000
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.