Ubiquilin 1 Polyclonal antibody

Ubiquilin 1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 23516-1-AP

Synonyms

DA41, DSK2, hPLIC 1, PLIC 1, PLIC1, ubiquilin 1, UBQLN1, XDRP1

---

Validation Data Gallery

• 

  WB analysis of rat brain using 23516-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23516-1-AP (Ubiquilin 1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 23516-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 23516-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 23516-1-AP

  Immunofluorescent analysis of PC-3 cells using 23516-1-AP (Ubiquilin 1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: rat brain tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IP: See 1 publications below

Product Information

23516-1-AP targets Ubiquilin 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ubiquilin 1 fusion protein Ag20252
• Full Name: ubiquilin 1
• Calculated Molecular Weight: 589 aa, 63 kDa
• Observed Molecular Weight: 63-71 kDa
• GenBank Accession Number: BC010066
• Gene Symbol: Ubiquilin 1
• Gene ID (NCBI): 29979
• RRID: AB_2879290
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBQLN1, also known as DSK2 or Ubiquilin 1, is an ubiquitin-like proteinis contains a N-terminal ubiquitin-like domain and a C-terminal ubiquitin-associated domain. They physically associate with both proteasomes and ubiquitin ligases, and thus are thought to functionally link the ubiquitination machinery to the proteasome to affect in vivo protein degradation.Ubiquilin has also been shown to modulate accumulation of presenilin proteins, and is found in lesions associated with Alzheimer's and Parkinson's disease. Two transcript variants encoding different isoforms have been found for this gene. Higher levels of ubiquilin-1 in the brain decreased malformation of the APP molecule which plays a key role in triggering Alzheimers disease.Conversely, lower levels of ubiquilin-1 in the brain were associated with increased malformation of APP (PMID: 21852239). This antibody detects 70kD band of UBQLN1.

Publications

Species	Application	Title
human	WB	Cancer Lett; HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1.; Authors - Zongfu Pan
human	IP	Biochim Biophys Acta Mol Basis Dis; Sec13 promotes glycolysis by inhibiting Ubqln1 mediated Pgm1 ubiquitination in ALI; Authors - Dongdong Wu
human	WB	Cancers (Basel); Knockdown of UBQLN1 Functions as a Strategy to Inhibit CRC Progression through the ERK-c-Myc Pathway; Authors - Ruoxuan Ni
human	WB	Oncotarget; The mir-675-5p regulates the progression and development of pancreatic cancer via the UBQLN1-ZEB1-mir200 axis.; Authors - Jue Wang
pig	WB	Mol Cell Proteomics; The mechano-ubiquitinome of articular cartilage: Differential ubiquitination and activation of a group of ER-associated DUBs and ER stress regulators; Authors - Nitchakarn Kaokhum
human		Cancers (Basel); Development of a Novel Circulating Autoantibody Biomarker Panel for the Identification of Patients with 'Actionable' Pulmonary Nodules; Authors - Claire Auger