Ubiquilin 2 Monoclonal antibody

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  WB analysis using 60495-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60495-1-Ig (Ubiquilin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 60495-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Ubiquilin 2 antibody (60495-1-Ig, Clone: 1A6D11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

Ubiquilin 2 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 60495-1-Ig

Clone No.1A6D11

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

UBQLN2, hPLIC2, hPLIC 2, Dsk2, Chap1

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, LNCaP cells, HeLa cells, MOLT-4 cells, pig brain tissue, rabbit brain tissue, rat brain tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60495-1-Ig targets Ubiquilin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig, rabbit samples.

• Tested Reactivity: human, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20251

  Product name: Recombinant human Ubiquilin 2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-624 aa of BC069237

  Sequence: MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS

• Full Name: ubiquilin 2
• Calculated Molecular Weight: 624 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC069237
• Gene Symbol: UBQLN2
• Gene ID (NCBI): 29978
• RRID: AB_3670264
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UHD9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.