Mouse Uromodulin Recombinant antibody, PBS Only

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  WB analysis using 85597-4-RR (same clone as 85597-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85597-4-RR (Umod antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 85597-4-RR (Uromodulin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse kidney using 85597-4-RR (same clone as 85597-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Uromodulin antibody (85597-4-RR, Clone: 242258H4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85597-4-PBS in a different storage buffer formulation.

Uromodulin Uni-rAb^TM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 85597-4-PBS

Clone No.242258H4

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Tamm-Horsfall urinary glycoprotein, THP, Umod, Uromodulin, secreted form

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Product Information

85597-4-PBS targets Uromodulin in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Uromodulin fusion protein Eg2748
• Full Name: Uromodulin
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: NM_009470.5
• Gene Symbol: Umod
• Gene ID (NCBI): 22242
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q91X17
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin is linked to water/electrolyte balance and kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)