Uteroglobin/CC10 Polyclonal antibody

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 26909-1-AP

Synonyms

SCGB1A1, Uteroglobin, Club cells 10 kDa secretory protein, Club cell phospholipid-binding protein, CCSP



Tested Applications

Positive WB detected inrat lung tissue
Positive IHC detected inhuman lung tissue, mouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26909-1-AP targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Uteroglobin/CC10 fusion protein Ag25530
Full Name secretoglobin, family 1A, member 1 (uteroglobin)
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC004481
Gene Symbol Uteroglobin
Gene ID (NCBI) 7356
RRIDAB_2880680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.

Protocols

Product Specific Protocols
WB protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
IHC protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
IF protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
FC protocol for Uteroglobin/CC10 antibody 26909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Immunol

Regulation of MHC Class I Expression in Lung Epithelial Cells during Inflammation.

Authors - Justine Mathé
ratIHC

Front Pediatr

Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.

Authors - Jun Wang
humanFC

Front Cell Infect Microbiol

BPIFA1 is a secreted biomarker of differentiating human airway epithelium

Authors - Clarissa Clifton