"V5-tag Antibodies" Comparison
View side-by-side comparison of V5-tag antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | Recombinant protein |
| Positive IP detected in | Recombinant protein protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14440-1-AP targets V5-tag in WB, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | V5 Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | V5 Tag |
| Gene ID (NCBI) | 4 |
| RRID | AB_2878059 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | V5TAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for V5-tag antibody 14440-1-AP | Download protocol |
| WB protocol for V5-tag antibody 14440-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The V5-tag Polyclonal Antibody (Cat# 14440-1-AP) has 110 citations in journals including Science, Nature, Nature Communications, Nature Cell Biology, Autophagy, Cell Reports, EMBO Journal, Nucleic Acids Research, Cell Death & Differentiation, and PNAS. Research fields span cancer biology, virology, autophagy, immunology, neuroscience, protein modification (ubiquitination/SUMOylation), fibrosis, and gene regulation. Cited applications include WB, IP, CoIP, IF, ChIP, and RIP.
| Species | Application | Title |
|---|---|---|
Science Boosting neuronal activity-driven mitochondrial DNA transcription improves cognition in aged mice | ||
Cancer Discov Mutational Signatures and Clonal Hematopoiesis in Intestinal Metaplasia across Countries with Varying Stomach Cancer Incidence. | ||
J Extracell Vesicles Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway. | ||
Autophagy MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis | ||
Autophagy USP5 attenuates NLRP3 inflammasome activation by promoting autophagic degradation of NLRP3. |
Reviews
What customers say
Overall, reviewers rate this V5 tag antibody favorably for Western blot, with several noting strong, specific, clear bands at dilutions from 1:1000 up to 1:20,000. Some users report good detection of tagged proteins after transfection. However, feedback is mixed for other applications: one reviewer found it did not work for immunofluorescence, another noted high background when used for IP, and one reported needing higher-than-recommended concentrations. A couple of users experienced nonspecific bands or weak signal in certain cell lines. In summary, it performs well for WB but may require optimization for IP and IF.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Lucy (Verified Customer) (02-26-2026) | Good detection of Tag upon transfection into two cell lines
|
Wenhui (Verified Customer) (12-03-2025) | The V5 antibody is working absolutely great. the bands are specific and clear. I can see a strong signaling at 1:1000 dilution.
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Nicole (Verified Customer) (08-04-2025) | did not work
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Zhongwen (Verified Customer) (09-25-2023) | The V5 antibodys shows clear band in western blots. But if you use this antibody for IP, the background is so strong that it is hard for you to find your band.
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Nick (Verified Customer) (04-20-2022) | Product for IP was used and had to use above the recommended concentrations
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Ben (Verified Customer) (10-12-2021) | Worked well, we got good images from it even at a very low dilution
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Daniela (Verified Customer) (01-08-2020) | In immunoblot of HeLa total cell lysate, this antibody gives several no specific bands in the negative control sample (cell lysate from no transfected cells). Whereas it doesn't recognize any specific protein in cell lysate from HeLa transfected with a plasmid for exogenous protein in frame with V5-tag. In immunofluorescence of transfected HeLa cells this antibody give a very faint signal.
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Erica (Verified Customer) (12-11-2019) | I used this antibody for westtern blot of V5 tagged protein and it worked very well. The signal is very strong and there isn't background. Highly recommend
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