V5-tag Polyclonal antibody

V5-tag Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, IF, IP, CoIP, ChIP, RIP, ELISA

Conjugate

Unconjugated

Publications

53

Reviews (5)

Cat no : 14440-1-AP

Synonyms

GKPIPNPLLGLDST, V5, V5 tag, V5-tag

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:32000 incubated at room temperature for 1.5 hours.

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  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Recombinant protein using 14440-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 14440-1-AP (V5-tag antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Recombinant protein using 14440-1-AP

  IP result of anti-V5-tag (IP:14440-1-AP, 3ug; Detection:14440-1-AP 1:20000) with Recombinant protein protein lysate 3000ug.

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Tested Applications

• Positive WB detected in: Recombinant protein
• Positive IP detected in: Recombinant protein protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 41 publications below
• IF: See 3 publications below
• IP: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Product Information

14440-1-AP targets V5-tag in WB, IF, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: V5-tag
• Calculated Molecular Weight: 1 kDa
• RRID: AB_2878059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publications

Species	Application	Title
	WB	Adv Sci (Weinh); RBMS1 Coordinates with the m6 A Reader YTHDF1 to Promote NSCLC Metastasis through Stimulating S100P Translation; Authors - Yu Sun
	WB	J Extracell Vesicles; Extracellular vesicles derived from oesophageal cancer containing P4HB promote muscle wasting via regulating PHGDH/Bcl-2/caspase-3 pathway.; Authors - Xiaohan Gao
	WB	Acta Pharm Sin B; Blockade of the deubiquitinating enzyme USP48 degrades oncogenic HMGA2 and inhibits colorectal cancer invasion and metastasis; Authors - Can Cheng
	WB	Sci Adv; The SUMO-interacting motif in NS2 promotes adaptation of avian influenza virus to mammals; Authors - Liuke Sun
	IF	Nat Commun; Dstyk mutation leads to congenital scoliosis-like vertebral malformations in zebrafish via dysregulated mTORC1/TFEB pathway.; Authors - Xianding Sun
	WB	Nucleic Acids Res; HSP90 and Aha1 modulate microRNA maturation through promoting the folding of Dicer1.; Authors - Xiaochuan Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zhongwen (Verified Customer) (09-25-2023)

The V5 antibodys shows clear band in western blots. But if you use this antibody for IP, the background is so strong that it is hard for you to find your band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Nick (Verified Customer) (04-20-2022)

Product for IP was used and had to use above the recommended concentrations

• Applications: Western Blot, Immunoprecipitation

FH

Ben (Verified Customer) (10-12-2021)

Worked well, we got good images from it even at a very low dilution

• Applications: Western Blot
• Primary Antibody Dilution: 1:20,000
• Cell Tissue Type: iAstrocyte

FH

Daniela (Verified Customer) (01-08-2020)

In immunoblot of HeLa total cell lysate, this antibody gives several no specific bands in the negative control sample (cell lysate from no transfected cells). Whereas it doesn't recognize any specific protein in cell lysate from HeLa transfected with a plasmid for exogenous protein in frame with V5-tag. In immunofluorescence of transfected HeLa cells this antibody give a very faint signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000 for immunoblot and 1:200 for immunofluorescence
• Cell Tissue Type: HeLa cells

FH

Erica (Verified Customer) (12-11-2019)

I used this antibody for westtern blot of V5 tagged protein and it worked very well. The signal is very strong and there isn't background. Highly recommend

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000 WB
• Cell Tissue Type: MEF