V5-tag Monoclonal antibody

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V5-tag Monoclonal Antibody for ELISA

Cat No. 66007-1-Ig

Clone No.2B7A9

Publications(38)

Reviews (2)

Host / Isotype

Mouse / IgG1

Reactivity

recombinant protein and More (1)

Applications

WB, IP, CoIP, RIP, ELISA

Synonyms

V5-tag

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Published Applications

• WB: See 19 publications below
• IP: See 4 publications below
• CoIP: See 3 publications below
• RIP: See 1 publications below

Product Information

66007-1-Ig targets V5-tag in WB, IP, CoIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: V5 Tag
• Calculated Molecular Weight: 1 kDa
• Gene Symbol: V5 Tag
• Gene ID (NCBI): 4
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: V5TAG
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Science; Structural insight into the SAM-mediated assembly of the mitochondrial TOM core complex.; Authors - Qiang Wang
	WB	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang
	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
		Dev Cell; Low-Level Saturated Fatty Acid Palmitate Benefits Liver Cells by Boosting Mitochondrial Metabolism via CDK1-SIRT3-CPT2 Cascade.; Authors - Lin Liu
		Endocrinology; PMID: 30059978; Authors - Jessica Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This V5 antibody detected my V5-tagged protein expressed in HEK293T cells. The band was very specific and strong.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

FH

Zehua (Verified Customer) (02-27-2019)

Works well for IB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T cell

V5-tag Antibody Western Blot,Immunoprecipitation, validation (1:2000 dilution) in 293T cell (Cat no:66007-1-Ig)