Filter:
Able AI
What's the best dilution for V5-tag in ?
Tested Applications
| Positive WB detected in | Transfected HEK-293 cells, Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81775-4-RR targets V5-tag in WB, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Reactivity | recombinant protein |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | V5 Tag |
| Calculated Molecular Weight | 1 kDa |
| Gene Symbol | V5 Tag |
| Gene ID (NCBI) | 4 |
| RRID | AB_3670504 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | V5TAG |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The antibody is developed against a synthetic 14 aa peptide (GKPIPNPLLGLDST), the V5 tag, which presents in many vectors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for V5-tag antibody 81775-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The V5-tag Recombinant monoclonal antibody (240529D8), catalog number 81775-4-RR, has 1 total citation. The cited publication appeared in Neuron (IF 16.9), published in 2026. The study investigated TMEM145 as a principal component of outer hair cell stereocilia, placing the research in the fields of neuroscience and auditory biology. The antibody was used in Western blot (WB) in this publication.
| Species | Application | Title |
|---|---|---|
