Validation Data Gallery
![The anti-V5 antibody [SV5-P-K] is a recombinant, monoclonal mouse IgG1, has very low background and detects even low amounts of V5-tagged proteins. Secondary antibody ChromoTek’s Alpaca anti-mouse IgG1 Alexa Fluor 488 Nano-Secondary.](https://www.ptglab.com/products/pictures/v5ab-2.jpg "The anti-V5 antibody [SV5-P-K] is a recombinant, monoclonal mouse IgG1, has very low background and detects even low amounts of V5-tagged proteins. Secondary antibody ChromoTek’s Alpaca anti-mouse IgG1 Alexa Fluor 488 Nano-Secondary.")
Product Information
Mouse monoclonal [SV5-P-K] to V5-tag
| Description | Mouse monoclonal [SV5-P-K] to V5-tag |
| Applications | WB |
| Specificity/Target | V5-Tag (GKPIPNPLLGLDST) |
| Recommended Dilution | Western Blot: 1:1,000 |
| Host/IsoType | Mouse/IgG1 kappa |
| Class | Monoclonal |
| Type | Primary Antibody |
| Format | Monoclonal mouse antibody |
| Clonality | Monoclonal |
| Clone No. | SV5-P-K |
| Immunogen | Pk (V5) epitope tag (GKPIPNPLLGLDST) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purified |
| RRID | AB_2868500 |
| Storage Buffer | PBS, preservative: 0.02 % Proclin |
| Storage Condition | Shipped on blue ice. Store at -20°C/-4°F. Stable for 1 year after shipment. |
| Size | 20 ug; 200 ug |
Documentation
| SDS |
|---|
| v5ab_SDS_V5-tag monoclonal antibody [SV5-P-K] (EN) |
| Datasheet |
|---|
| V5-tag Monoclonal antibody (SV5-P-K) Datasheet |
Publications
| Application | Title |
|---|---|
Metab Eng Combinatorial optimization and spatial remodeling of CYPs to control product profile | |
Dev Comp Immunol The DNA cytosine-5-methyltransferase 3 (DNMT3) involved in regulation of CgIL-17 expression in the immune response of oyster Crassostrea gigas. | |
PLoS Pathog Polar localization of CheO under hypoxia promotes Campylobacter jejuni chemotactic behavior within host | |
J Med Virol SARS-CoV-2 NSP7 inhibits type I and III IFN production by targeting the RIG-I/MDA5, TRIF, and STING signaling pathways | |
Sci Adv A nucleus-encoded dynamin-like protein controls endosymbiont division in the trypanosomatid Angomonas deanei |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Magdalena (Verified Customer) (02-12-2025) | I tested this antibody for Westernblto of HEK cell lysate transfected with a V5-tagged protein, and its V5-Trap pulldown. I didn't detect anything with the 1:5000 dilution, but haven't tried higher antibody concentrations.
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