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VAChT Polyclonal antibody

VAChT Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27303-1-AP

Synonyms

SLC18A3, VACHT

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VAChT Polyclonal antibody (27303-1-AP)

    WB analysis using 27303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27303-1-AP (VAChT antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27303-1-AP targets VAChT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VAChT fusion protein Ag25153
Full Name solute carrier family 18 (vesicular acetylcholine), member 3
Calculated Molecular Weight 532 aa, 57 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007765
Gene Symbol SLC18A3
Gene ID (NCBI) 6572
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC18A3 also known as VAChT, is the vesicular amine transporter family member. The SLC18A3 gene encodes a transmembrane protein that transports acetylcholine (ACh) into presynaptic secretory vesicles for release at cholinergic nerve endings in the central and peripheral nervous systems. Mutations in SLC18A3 are associated with congenital myasthenic syndrome(PMID: 27590285). The 68-70 kDa mature glycosylated form of VAChT can be detected (PMID: 14705140).

Protocols

Product Specific Protocols
WB protocol for VAChT antibody 27303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols