VAMP2 Polyclonal antibody

• 

VAMP2 Polyclonal Antibody for WB, ELISA

Cat No. 30001-0-AP

Publications(4)

Host / Isotype

Goat / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SYB2, Synaptobrevin 2, VAMP 2, VAMP2

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Tested Applications

• Positive WB detected in: Mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• ELISA: See 3 publications below

Product Information

30001-0-AP targets VAMP2 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Goat / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: vesicle-associated membrane protein 2 (synaptobrevin 2)
• Calculated Molecular Weight: 13 kDa
• GenBank Accession Number: NM_014232
• Gene Symbol: VAMP2
• Gene ID (NCBI): 6844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P63027
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP2 (vesicle-associated membrane protein 2), also named as synaptobrevin 2, is a member of the SNARE (soluble NSF-attachment protein receptor) family of proteins. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP2 consists of a short N-terminal sequence, a SNARE motif, and a C-terminal transmembrane region. It is required for fast calcium-triggered synaptic vesicle fusion. VAMP2 forms a stable complex with STX1 (syntaxin 1) and SNAP25 (synaptosomal-associated protein 25) during synaptic vesicle fusion (PMID: 16793874). It also forms a distinct complex with synaptophysin. VAMP2 is expressed in nervous system and some non-neuronal tissues, such as skeletal muscle (PMID: 18570252).

Publications

Species	Application	Title
human	ELISA	J Cell Biochem; Un-nicked BoNT/B activity in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; TCEP treatment reduces proteolytic activity of BoNT/B in human neuronal SHSY-5Y cells.; Authors - Shi Xuerong X
human	ELISA	J Cell Biochem; The biological activity of ubiquitinated BoNT/B light chain in vitro and in human SHSY-5Y neuronal cells.; Authors - Shi Xuerong X
human	WB	Mol Neurobiol; L116 Deletion in CSPα Promotes α-Synuclein Aggregation and Neurodegeneration; Authors - Tao Guo