Various lysates were subjected to SDS PAGE followed by western blot with RMX00044 (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00044 (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of THP-1 using RMX00044
IP result of anti-VAMP8 (IP:RMX00044, 4ug; Detection:RMX00044 1:10000) with THP-1 cells lysate 1800 ug.
IP result of anti-VAMP8 (IP:RMX00044, 4ug; Detection:RMX00044 1:10000) with THP-1 cells lysate 1800 ug.
IHC staining of human placenta using RMX00044
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using RMX00044
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using RMX00044
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAMP8 antibody (RMX00044) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAMP8 antibody (RMX00044) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
HL-60 cells, THP-1 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected in
THP-1 cells
Positive IHC detected in
human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
RMX00044 targets VAMP8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
VAMP8, also named endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy by directly controlling the fusion of autophagosomes with the lysosomal membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.
Various lysates were subjected to SDS PAGE followed by western blot with RMX00044 (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human placenta using RMX00044
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using RMX00044
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using RMX00044 (VAMP8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IP Figures
IP experiment of THP-1 using RMX00044
IP result of anti-VAMP8 (IP:RMX00044, 4ug; Detection:RMX00044 1:10000) with THP-1 cells lysate 1800 ug.
IF/ICC Figures
IF Staining of HeLa using RMX00044
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VAMP8 antibody (RMX00044) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
VAMP8 Multi-Recombinant antibody
Catalog Number
RMX00044
Citations
-
Dilutions
WB : 1:5000-1:50000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:500-1:2000 IF/ICC : 1:200-1:800
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
